5 Citations (Scopus)

Abstract

Objectives: To identify the relationships between key components of the proliferative and apoptotic pathways in bladder tumors. Methods: A tissue array of 88 bladder tumors was assembled. Immunohistochemical analyses were used to investigate the relationship between nine different parameters: stage, proliferation (Ki67), apoptosis (in situ DNA nick end labeling), the anti-apoptotic protein Bcl-2, tumor suppressors p53 and retinoblastoma protein (Rb), the Rb-related protein p130, cyclin E, and the cyclin-dependent kinase inhibitor p27. The protein expression in each tumor was reported as the percentage of positively staining cells. Results: The analysis focused on Stage 1 to 3 tumors. Analysis found that p53 expression increased progressively with stage, and Rb and p27 decreased with increasing stage. Overall, the cyclin E levels correlated with the proliferative index. Cyclin E levels were low in Stage 1 tumors and elevated in Stage 2 tumors, but were decreased in Stage 3 tumors. Multivariate analysis uncovered a correlation between cyclin E and proliferation (Ki67) and a weak correlation between p53 and Bcl-2 and between p27 and Rb. A strong correlation was found between the expression of p53 and p130, which was apparent in Stages 1 and 3, but not in Stage 2. Furthermore, high levels of p130 protein were detected primarily in the cytoplasm. Conclusions: These results suggest a novel p53/p130 axis in bladder tumors.

Original languageEnglish (US)
Pages (from-to)608-612
Number of pages5
JournalUrology
Volume70
Issue number3
DOIs
StatePublished - Sep 2007

Fingerprint

Cyclin E
Urinary Bladder Neoplasms
Retinoblastoma Protein
Neoplasms
Cyclin-Dependent Kinase Inhibitor p27
Tumor Suppressor Proteins
Single-Stranded DNA Breaks
Apoptosis Regulatory Proteins
In Situ Nick-End Labeling
Cytoplasm
Proteins
Multivariate Analysis
Apoptosis
Staining and Labeling

ASJC Scopus subject areas

  • Urology

Cite this

Novel p53/p130 Axis in Bladder Tumors. / Mudryj, Maria; Reay, Elizabeth; Beckett, Laurel A; Dandekar, Satya; deVere White, Ralph W; Gandour-Edwards, Regina F.

In: Urology, Vol. 70, No. 3, 09.2007, p. 608-612.

Research output: Contribution to journalArticle

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title = "Novel p53/p130 Axis in Bladder Tumors",
abstract = "Objectives: To identify the relationships between key components of the proliferative and apoptotic pathways in bladder tumors. Methods: A tissue array of 88 bladder tumors was assembled. Immunohistochemical analyses were used to investigate the relationship between nine different parameters: stage, proliferation (Ki67), apoptosis (in situ DNA nick end labeling), the anti-apoptotic protein Bcl-2, tumor suppressors p53 and retinoblastoma protein (Rb), the Rb-related protein p130, cyclin E, and the cyclin-dependent kinase inhibitor p27. The protein expression in each tumor was reported as the percentage of positively staining cells. Results: The analysis focused on Stage 1 to 3 tumors. Analysis found that p53 expression increased progressively with stage, and Rb and p27 decreased with increasing stage. Overall, the cyclin E levels correlated with the proliferative index. Cyclin E levels were low in Stage 1 tumors and elevated in Stage 2 tumors, but were decreased in Stage 3 tumors. Multivariate analysis uncovered a correlation between cyclin E and proliferation (Ki67) and a weak correlation between p53 and Bcl-2 and between p27 and Rb. A strong correlation was found between the expression of p53 and p130, which was apparent in Stages 1 and 3, but not in Stage 2. Furthermore, high levels of p130 protein were detected primarily in the cytoplasm. Conclusions: These results suggest a novel p53/p130 axis in bladder tumors.",
author = "Maria Mudryj and Elizabeth Reay and Beckett, {Laurel A} and Satya Dandekar and {deVere White}, {Ralph W} and Gandour-Edwards, {Regina F}",
year = "2007",
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T1 - Novel p53/p130 Axis in Bladder Tumors

AU - Mudryj, Maria

AU - Reay, Elizabeth

AU - Beckett, Laurel A

AU - Dandekar, Satya

AU - deVere White, Ralph W

AU - Gandour-Edwards, Regina F

PY - 2007/9

Y1 - 2007/9

N2 - Objectives: To identify the relationships between key components of the proliferative and apoptotic pathways in bladder tumors. Methods: A tissue array of 88 bladder tumors was assembled. Immunohistochemical analyses were used to investigate the relationship between nine different parameters: stage, proliferation (Ki67), apoptosis (in situ DNA nick end labeling), the anti-apoptotic protein Bcl-2, tumor suppressors p53 and retinoblastoma protein (Rb), the Rb-related protein p130, cyclin E, and the cyclin-dependent kinase inhibitor p27. The protein expression in each tumor was reported as the percentage of positively staining cells. Results: The analysis focused on Stage 1 to 3 tumors. Analysis found that p53 expression increased progressively with stage, and Rb and p27 decreased with increasing stage. Overall, the cyclin E levels correlated with the proliferative index. Cyclin E levels were low in Stage 1 tumors and elevated in Stage 2 tumors, but were decreased in Stage 3 tumors. Multivariate analysis uncovered a correlation between cyclin E and proliferation (Ki67) and a weak correlation between p53 and Bcl-2 and between p27 and Rb. A strong correlation was found between the expression of p53 and p130, which was apparent in Stages 1 and 3, but not in Stage 2. Furthermore, high levels of p130 protein were detected primarily in the cytoplasm. Conclusions: These results suggest a novel p53/p130 axis in bladder tumors.

AB - Objectives: To identify the relationships between key components of the proliferative and apoptotic pathways in bladder tumors. Methods: A tissue array of 88 bladder tumors was assembled. Immunohistochemical analyses were used to investigate the relationship between nine different parameters: stage, proliferation (Ki67), apoptosis (in situ DNA nick end labeling), the anti-apoptotic protein Bcl-2, tumor suppressors p53 and retinoblastoma protein (Rb), the Rb-related protein p130, cyclin E, and the cyclin-dependent kinase inhibitor p27. The protein expression in each tumor was reported as the percentage of positively staining cells. Results: The analysis focused on Stage 1 to 3 tumors. Analysis found that p53 expression increased progressively with stage, and Rb and p27 decreased with increasing stage. Overall, the cyclin E levels correlated with the proliferative index. Cyclin E levels were low in Stage 1 tumors and elevated in Stage 2 tumors, but were decreased in Stage 3 tumors. Multivariate analysis uncovered a correlation between cyclin E and proliferation (Ki67) and a weak correlation between p53 and Bcl-2 and between p27 and Rb. A strong correlation was found between the expression of p53 and p130, which was apparent in Stages 1 and 3, but not in Stage 2. Furthermore, high levels of p130 protein were detected primarily in the cytoplasm. Conclusions: These results suggest a novel p53/p130 axis in bladder tumors.

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