Novel mechanisms revealed in the trachea transcriptome of resistant and susceptible chicken lines following infection with newcastle disease virus

Melissa S. Deist, Rodrigo A Gallardo, David A. Bunn, Terra R. Kelly, Jack C.M. Dekkers, Huaijun Zhou, Susan J. Lamonta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Newcastle disease virus (NDV) has a devastating impact on poultry production in developing countries. This study examined the transcriptome of tracheal epithelial cells from two inbred chicken lines that differ in NDV susceptibility after challenge with a high-titer inoculum of lentogenic NDV. The Fayoumi line had a significantly lower NDV load postchallenge than the Leghorn line, demonstrating the Fayoumi line's classification as a relatively NDV-resistant breed. Examination of the trachea transcriptome showed a large increase in immune cell infiltration in the trachea in both lines at all times postinfection. The pathways conserved across lines and at all three time points postinfection included iCOS-iCOSL signaling in T helper cells, NF-κB signaling, the role of nuclear factor of activated T cells in the regulation of the immune response, calcium-induced T lymphocyte apoptosis, phospholipase C signaling, and CD28 signaling in T helper cells. Although shared pathways were seen in the Fayoumi and Leghorn lines, each line showed unique responses as well. The downregulation of collagen and the activation of eukaryotic translation initiation factor 2 signaling in the Fayoumis relative to the Leghorns at 2 days postinfection may contribute to the resistance phenotype seen in the Fayoumis. This study provides a further understanding of host-pathogen interactions which could improve vaccine efficacy and, in combination with genome-wide association studies, has the potential to advance strategies for breeding chickens with enhanced resistance to NDV.

Original languageEnglish (US)
Article numbere00027
JournalClinical and Vaccine Immunology
Volume24
Issue number5
DOIs
StatePublished - May 1 2017

Fingerprint

Newcastle disease virus
Trachea
Viruses
Transcriptome
Chickens
Infection
Helper-Inducer T-Lymphocytes
Prokaryotic Initiation Factor-2
Eukaryotic Initiation Factor-2
NFATC Transcription Factors
Eukaryotic Initiation Factors
Host-Pathogen Interactions
Poultry
T-cells
Genome-Wide Association Study
Disease Susceptibility
Type C Phospholipases
Pathogens
Infiltration
Developing countries

Keywords

  • Chicken
  • Collagen
  • EIF2 signaling
  • Newcastle disease virus
  • RNA-seq
  • Trachea

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Novel mechanisms revealed in the trachea transcriptome of resistant and susceptible chicken lines following infection with newcastle disease virus. / Deist, Melissa S.; Gallardo, Rodrigo A; Bunn, David A.; Kelly, Terra R.; Dekkers, Jack C.M.; Zhou, Huaijun; Lamonta, Susan J.

In: Clinical and Vaccine Immunology, Vol. 24, No. 5, e00027, 01.05.2017.

Research output: Contribution to journalArticle

Deist, Melissa S. ; Gallardo, Rodrigo A ; Bunn, David A. ; Kelly, Terra R. ; Dekkers, Jack C.M. ; Zhou, Huaijun ; Lamonta, Susan J. / Novel mechanisms revealed in the trachea transcriptome of resistant and susceptible chicken lines following infection with newcastle disease virus. In: Clinical and Vaccine Immunology. 2017 ; Vol. 24, No. 5.
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