Abstract
Acute lymphoblastic leukemia (ALL) with translocation t(4;11) is found in 60-85% of infants with ALL and is often refractory to conventional chemotherapeutics after relapse. Using the t(4;11) ALL line SEM, we evaluated chemotherapy resistance in NOD.CB17-Prkdcscid/J mice. SEM cells were injected into the tail vein and engraftment was monitored by flow cytometry. Once engraftment was observed, mice were injected intraperitoneally with phosphate-buffered saline (PBS), or vincristine (0.5. mg/kg body weight) three times per week for 4. weeks (n=8 per group). The level of P-glycoprotein in SEM cells was increased 3-fold by vincristine treatment compared to PBS-treated mice. Survival curves showed that leukemia cell growth was initially delayed by vincristine treatment, but the mice eventually succumbed to disease. These data describe a novel inducible model for investigating multi-drug resistance mechanisms in high-risk t(4;11) ALL.
Original language | English (US) |
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Pages (from-to) | 49-54 |
Number of pages | 6 |
Journal | Cancer Letters |
Volume | 296 |
Issue number | 1 |
DOIs | |
State | Published - Oct 2010 |
Keywords
- Leukemia
- Multi-drug resistance
- P-glycoprotein
- Translocation
ASJC Scopus subject areas
- Cancer Research
- Oncology