Novel high-sensitivity fluorescence polarization reader

C. C. Hoyt, Richard M Levenson, P. R. Banks

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Advances in functional genomics and combinatorial chemistry require improvements in current drug screening instrumentation: a drug discovery bottleneck has been generated by the inability of current technology to expeditiously explore such combinatorial libraries of drug candidates for new target identification. To alleviate this problem, the sample-throughput of screening instrumentation needs to improve without sacrificing and precision-both required for cost-effective, accurate lead identification. We have developed a new fluorescence polarization (FP) reader suitable for high-throughput screening (HTS) and ultra-HTS whose assay-performance and sample-throughput are both considerably improved over present state-of-the-art instrumentation. The Symmetry TM reader possesses a number of features that differ from conventional HTS FP readers. These include: laser-based excitation; liquid crystal polarization optics that rapidly and accurately measure polarization states; and CCD detectors to capture emission from multiple wells. We show that the performance in assays relevant to the drug discovery process, such as G- protein coupled receptor-based assays, is significantly enhanced due to a dramatic improvement in precision. Furthermore, the CCD-detection system used can substantially improve sample throughput compared to sequential readers while maintaining high performance.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsG.E. Cohn
Pages28-32
Number of pages5
Volume4255
DOIs
StatePublished - 2001
Externally publishedYes
EventClinical Diagnostic Systems - San Jose, CA, United States
Duration: Jan 21 2001Jan 22 2001

Other

OtherClinical Diagnostic Systems
CountryUnited States
CitySan Jose, CA
Period1/21/011/22/01

Keywords

  • Fluorescence polarization
  • High-throughput drug screening
  • Liquid crystals

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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