Novel heterotrimeric kinesin-related protein purified from sea urchin eggs

D. G. Cole, S. W. Chinn, K. P. Wedaman, K. Hall, T. Vuong, J. M. Scholey

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

KINESIN heavy chain and kinesin-related polypeptides (KRPs) comprise a family of motor proteins with diverse intracellular transport functions1-7. Using pan-kinesin peptide antibodies that react with these proteins8,9, we have previously purified from sea urchin eggs a trimeric microtubule-binding and bundling protein, KRP(85/95) (ref. 8) comprising subunits of Mr 115,000 (115K), 95K and 85K. We report here that kinesin-related genes encode the 85K and 95K subunits, and that the protein can be immunoprecipitated from cytosol as a trimeric complex using an 85K monoclonal antibody. We also find that purified KRP(85/95) directs movements towards the 'plus' ends of microtubules. To our knowledge, this protein is the first kinesin-related motor to be purified from its natural host cell in a native multimeric state.

Original languageEnglish (US)
Pages (from-to)268-270
Number of pages3
JournalNature
Volume366
Issue number6452
StatePublished - Nov 18 1993

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Kinesin
Sea Urchins
Eggs
Peptides
Proteins
Microtubules
Protein Subunits
Cytosol
Carrier Proteins
Monoclonal Antibodies
Antibodies
Genes

ASJC Scopus subject areas

  • General

Cite this

Cole, D. G., Chinn, S. W., Wedaman, K. P., Hall, K., Vuong, T., & Scholey, J. M. (1993). Novel heterotrimeric kinesin-related protein purified from sea urchin eggs. Nature, 366(6452), 268-270.

Novel heterotrimeric kinesin-related protein purified from sea urchin eggs. / Cole, D. G.; Chinn, S. W.; Wedaman, K. P.; Hall, K.; Vuong, T.; Scholey, J. M.

In: Nature, Vol. 366, No. 6452, 18.11.1993, p. 268-270.

Research output: Contribution to journalArticle

Cole, DG, Chinn, SW, Wedaman, KP, Hall, K, Vuong, T & Scholey, JM 1993, 'Novel heterotrimeric kinesin-related protein purified from sea urchin eggs', Nature, vol. 366, no. 6452, pp. 268-270.
Cole DG, Chinn SW, Wedaman KP, Hall K, Vuong T, Scholey JM. Novel heterotrimeric kinesin-related protein purified from sea urchin eggs. Nature. 1993 Nov 18;366(6452):268-270.
Cole, D. G. ; Chinn, S. W. ; Wedaman, K. P. ; Hall, K. ; Vuong, T. ; Scholey, J. M. / Novel heterotrimeric kinesin-related protein purified from sea urchin eggs. In: Nature. 1993 ; Vol. 366, No. 6452. pp. 268-270.
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