Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas: Identification, prevalence, and risk factors

Ryan M. Troyer, Julia A. Beatty, Kathryn R. Stutzman-Rodriguez, Scott Carver, Caitlin C. Lozano, Justin S. Lee, Michael R. Lappin, Seth P D Riley, Laurel E K Serieys, Kenneth A. Logan, Linda L. Sweanor, Walter M Boyce, T. Winston Vickers, Roy McBride, Kevin R. Crooks, Jesse S. Lewis, Mark W. Cunningham, Joel Rovnak, Sandra L. Quackenbush, Sue VandeWoude

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Gammaherpesviruses (GHVs) are a diverse and rapidly expanding group of viruses associated with a variety of disease conditions in humans and animals. To identify felid GHVs, we screened domestic cat (Felis catus), bobcat (Lynx rufus), and puma (Puma concolor) blood cell DNA samples from California, Colorado, and Florida using a degenerate pan-GHV PCR. Additional pan-GHV and long-distance PCRs were used to sequence a contiguous 3.4-kb region of each putative virus species, including partial glycoprotein B and DNA polymerase genes. We identified three novel GHVs, each present predominantly in one felid species: Felis catus GHV 1 (FcaGHV1) in domestic cats, Lynx rufus GHV 1 (LruGHV1) in bobcats, and Puma concolor GHV 1 (PcoGHV1) in pumas. To estimate infection prevalence, we developed real-time quantitative PCR assays for each virus and screened additional DNA samples from all three species (n=282). FcaGHV1 was detected in 16% of domestic cats across all study sites. LruGHV1 was detected in 47% of bobcats and 13% of pumas across all study sites, suggesting relatively common interspecific transmission. PcoGHV1 was detected in 6% of pumas, all from a specific region of Southern California. The risk of infection for each host varied with geographic location. Age was a positive risk factor for bobcat LruGHV1 infection, and age and being male were risk factors for domestic cat FcaGHV1 infection. Further characterization of these viruses may have significant health implications for domestic cats and may aid studies of free-ranging felid ecology.

Original languageEnglish (US)
Pages (from-to)3914-3924
Number of pages11
JournalJournal of Virology
Volume88
Issue number8
DOIs
StatePublished - 2014

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Lynx
Puma
Lynx rufus
Cats
risk factors
cats
Puma concolor
Felidae
Viruses
viruses
Infection
infection
Geographic Locations
Polymerase Chain Reaction
DNA
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
blood cells
Ecology
glycoproteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Troyer, R. M., Beatty, J. A., Stutzman-Rodriguez, K. R., Carver, S., Lozano, C. C., Lee, J. S., ... VandeWoude, S. (2014). Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas: Identification, prevalence, and risk factors. Journal of Virology, 88(8), 3914-3924. https://doi.org/10.1128/JVI.03405-13

Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas : Identification, prevalence, and risk factors. / Troyer, Ryan M.; Beatty, Julia A.; Stutzman-Rodriguez, Kathryn R.; Carver, Scott; Lozano, Caitlin C.; Lee, Justin S.; Lappin, Michael R.; Riley, Seth P D; Serieys, Laurel E K; Logan, Kenneth A.; Sweanor, Linda L.; Boyce, Walter M; Winston Vickers, T.; McBride, Roy; Crooks, Kevin R.; Lewis, Jesse S.; Cunningham, Mark W.; Rovnak, Joel; Quackenbush, Sandra L.; VandeWoude, Sue.

In: Journal of Virology, Vol. 88, No. 8, 2014, p. 3914-3924.

Research output: Contribution to journalArticle

Troyer, RM, Beatty, JA, Stutzman-Rodriguez, KR, Carver, S, Lozano, CC, Lee, JS, Lappin, MR, Riley, SPD, Serieys, LEK, Logan, KA, Sweanor, LL, Boyce, WM, Winston Vickers, T, McBride, R, Crooks, KR, Lewis, JS, Cunningham, MW, Rovnak, J, Quackenbush, SL & VandeWoude, S 2014, 'Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas: Identification, prevalence, and risk factors', Journal of Virology, vol. 88, no. 8, pp. 3914-3924. https://doi.org/10.1128/JVI.03405-13
Troyer, Ryan M. ; Beatty, Julia A. ; Stutzman-Rodriguez, Kathryn R. ; Carver, Scott ; Lozano, Caitlin C. ; Lee, Justin S. ; Lappin, Michael R. ; Riley, Seth P D ; Serieys, Laurel E K ; Logan, Kenneth A. ; Sweanor, Linda L. ; Boyce, Walter M ; Winston Vickers, T. ; McBride, Roy ; Crooks, Kevin R. ; Lewis, Jesse S. ; Cunningham, Mark W. ; Rovnak, Joel ; Quackenbush, Sandra L. ; VandeWoude, Sue. / Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas : Identification, prevalence, and risk factors. In: Journal of Virology. 2014 ; Vol. 88, No. 8. pp. 3914-3924.
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