Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium

Lauren L. Daniele, Ralf H. Adams, Diane E. Durante, Edward N Pugh Jr, Nancy J. Philp

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Junction adhesion molecules-A, -B, and -C (Jams) are cell surface glycoproteins that have been shown to play an important role in the assembly and maintenance of tight junctions and in the establishment of epithelial cell polarity. Recent studies reported that Jam-C mRNA was increased threefold in the all-cone retina of the Nrl-/- mouse, suggesting that Jam-C is required for maturation and polarization of cone photoreceptors cells. We examined the expression of Jams in the mouse retina by using confocal immunofluorescence localization. Jam-C was detected in tight junctions of retinal pigment epithelium (RPE) and at the outer limiting membrane (OLM) in the specialized adherens junctions between Müller and photoreceptor cells. Additionally, Jam-C labeling was observed in the long apical processes of Müller and RPE cells that extend between the inner segments and outer segments of photoreceptors, respectively. Jam-B was also detected at the OLM. In the developing retina, Jam-B and -C were detected at the apical junctions of embryonic retinal neuroepithelia, suggesting a role for Jams in retinogenesis. In eyes from Jam-C-/- mice, retinal lamination, polarity, and photoreceptor morphology appeared normal. Although Jam-A was not detected at the OLM in wild-type retinas, it was present at the OLM in retinas of Jam-C -/- mice. These findings indicate that up-regulation of Jam-A in the retina compensates for the loss Jam-C. The nonclassical distribution of Jam-C in the apical membranes of Müller cells and RPE suggests that Jam-C has a novel function in the retina.

Original languageEnglish (US)
Pages (from-to)166-176
Number of pages11
JournalJournal of Comparative Neurology
Volume505
Issue number2
DOIs
StatePublished - Nov 10 2007
Externally publishedYes

Fingerprint

Junctional Adhesion Molecule C
Retinal Pigment Epithelium
Retina
Retinal Cone Photoreceptor Cells
Membranes
Tight Junctions
Junctional Adhesion Molecules
Adherens Junctions
Cell Polarity
Photoreceptor Cells
Membrane Glycoproteins
Fluorescent Antibody Technique
Up-Regulation
Epithelial Cells
Maintenance
Cell Membrane
Messenger RNA

Keywords

  • Adherens junction
  • Cell polarity
  • Jam-B
  • Jam-C
  • Outer limiting membrane
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium. / Daniele, Lauren L.; Adams, Ralf H.; Durante, Diane E.; Pugh Jr, Edward N; Philp, Nancy J.

In: Journal of Comparative Neurology, Vol. 505, No. 2, 10.11.2007, p. 166-176.

Research output: Contribution to journalArticle

Daniele, Lauren L. ; Adams, Ralf H. ; Durante, Diane E. ; Pugh Jr, Edward N ; Philp, Nancy J. / Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium. In: Journal of Comparative Neurology. 2007 ; Vol. 505, No. 2. pp. 166-176.
@article{e50116da1e424bb3be05184977a943cd,
title = "Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium",
abstract = "Junction adhesion molecules-A, -B, and -C (Jams) are cell surface glycoproteins that have been shown to play an important role in the assembly and maintenance of tight junctions and in the establishment of epithelial cell polarity. Recent studies reported that Jam-C mRNA was increased threefold in the all-cone retina of the Nrl-/- mouse, suggesting that Jam-C is required for maturation and polarization of cone photoreceptors cells. We examined the expression of Jams in the mouse retina by using confocal immunofluorescence localization. Jam-C was detected in tight junctions of retinal pigment epithelium (RPE) and at the outer limiting membrane (OLM) in the specialized adherens junctions between M{\"u}ller and photoreceptor cells. Additionally, Jam-C labeling was observed in the long apical processes of M{\"u}ller and RPE cells that extend between the inner segments and outer segments of photoreceptors, respectively. Jam-B was also detected at the OLM. In the developing retina, Jam-B and -C were detected at the apical junctions of embryonic retinal neuroepithelia, suggesting a role for Jams in retinogenesis. In eyes from Jam-C-/- mice, retinal lamination, polarity, and photoreceptor morphology appeared normal. Although Jam-A was not detected at the OLM in wild-type retinas, it was present at the OLM in retinas of Jam-C -/- mice. These findings indicate that up-regulation of Jam-A in the retina compensates for the loss Jam-C. The nonclassical distribution of Jam-C in the apical membranes of M{\"u}ller cells and RPE suggests that Jam-C has a novel function in the retina.",
keywords = "Adherens junction, Cell polarity, Jam-B, Jam-C, Outer limiting membrane, Retina",
author = "Daniele, {Lauren L.} and Adams, {Ralf H.} and Durante, {Diane E.} and {Pugh Jr}, {Edward N} and Philp, {Nancy J.}",
year = "2007",
month = "11",
day = "10",
doi = "10.1002/cne.21489",
language = "English (US)",
volume = "505",
pages = "166--176",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium

AU - Daniele, Lauren L.

AU - Adams, Ralf H.

AU - Durante, Diane E.

AU - Pugh Jr, Edward N

AU - Philp, Nancy J.

PY - 2007/11/10

Y1 - 2007/11/10

N2 - Junction adhesion molecules-A, -B, and -C (Jams) are cell surface glycoproteins that have been shown to play an important role in the assembly and maintenance of tight junctions and in the establishment of epithelial cell polarity. Recent studies reported that Jam-C mRNA was increased threefold in the all-cone retina of the Nrl-/- mouse, suggesting that Jam-C is required for maturation and polarization of cone photoreceptors cells. We examined the expression of Jams in the mouse retina by using confocal immunofluorescence localization. Jam-C was detected in tight junctions of retinal pigment epithelium (RPE) and at the outer limiting membrane (OLM) in the specialized adherens junctions between Müller and photoreceptor cells. Additionally, Jam-C labeling was observed in the long apical processes of Müller and RPE cells that extend between the inner segments and outer segments of photoreceptors, respectively. Jam-B was also detected at the OLM. In the developing retina, Jam-B and -C were detected at the apical junctions of embryonic retinal neuroepithelia, suggesting a role for Jams in retinogenesis. In eyes from Jam-C-/- mice, retinal lamination, polarity, and photoreceptor morphology appeared normal. Although Jam-A was not detected at the OLM in wild-type retinas, it was present at the OLM in retinas of Jam-C -/- mice. These findings indicate that up-regulation of Jam-A in the retina compensates for the loss Jam-C. The nonclassical distribution of Jam-C in the apical membranes of Müller cells and RPE suggests that Jam-C has a novel function in the retina.

AB - Junction adhesion molecules-A, -B, and -C (Jams) are cell surface glycoproteins that have been shown to play an important role in the assembly and maintenance of tight junctions and in the establishment of epithelial cell polarity. Recent studies reported that Jam-C mRNA was increased threefold in the all-cone retina of the Nrl-/- mouse, suggesting that Jam-C is required for maturation and polarization of cone photoreceptors cells. We examined the expression of Jams in the mouse retina by using confocal immunofluorescence localization. Jam-C was detected in tight junctions of retinal pigment epithelium (RPE) and at the outer limiting membrane (OLM) in the specialized adherens junctions between Müller and photoreceptor cells. Additionally, Jam-C labeling was observed in the long apical processes of Müller and RPE cells that extend between the inner segments and outer segments of photoreceptors, respectively. Jam-B was also detected at the OLM. In the developing retina, Jam-B and -C were detected at the apical junctions of embryonic retinal neuroepithelia, suggesting a role for Jams in retinogenesis. In eyes from Jam-C-/- mice, retinal lamination, polarity, and photoreceptor morphology appeared normal. Although Jam-A was not detected at the OLM in wild-type retinas, it was present at the OLM in retinas of Jam-C -/- mice. These findings indicate that up-regulation of Jam-A in the retina compensates for the loss Jam-C. The nonclassical distribution of Jam-C in the apical membranes of Müller cells and RPE suggests that Jam-C has a novel function in the retina.

KW - Adherens junction

KW - Cell polarity

KW - Jam-B

KW - Jam-C

KW - Outer limiting membrane

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=35548938609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35548938609&partnerID=8YFLogxK

U2 - 10.1002/cne.21489

DO - 10.1002/cne.21489

M3 - Article

C2 - 17853450

AN - SCOPUS:35548938609

VL - 505

SP - 166

EP - 176

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 2

ER -