Novel angiotensin-I-converting enzyme inhibitory peptides derived from recombinant human α(s1)-casein expressed in Escherichia coli

Yoo Kyeong Kim, Sun Yoon, Dae Yeul Yu, Bo Lönnerdal, Bong Hyun Chung

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Recombinant human α(s1)-casein expressed in Escherichia coli was purified and digested with trypsin in an attempt to find peptides with angiotensin-I-converting enzyme (ACE) inhibitory activity. Three novel ACE inhibitory peptides, A-II. B-II and C, were isolated and their amino acid sequences identified as Tyr-Pro-Glu-Arg (residues 8-11), Tyr-Tyr-Pro-Gln-Ile-Met-Gln-Tyr (residues 136-143) and Asn-Asn-Val-Met-Leu-Gln-Trp (residues 164-170) respectively. ACE inhibitory activities were measured for the corresponding synthetic peptides, and the ACE IC50 (the amount of peptide causing 50% inhibition of ACE activity) values of A-II, B-II and C estimated to be 132.5, 24.8 and 41.0 μmol/l respectively. Peptides A-II and C were resistant to further digestion by pepsin, whereas peptide B-II was hydrolysed. All three peptides were resistant to digestion by chymotrypsin. These ACE inhibitory peptides may prove useful for oral administration in the treatment of hypertension.

Original languageEnglish (US)
Pages (from-to)431-439
Number of pages9
JournalJournal of Dairy Research
Volume66
Issue number3
DOIs
StatePublished - Aug 1999

Fingerprint

peptidyl-dipeptidase A
Peptidyl-Dipeptidase A
Caseins
casein
peptides
Escherichia coli
Peptides
Digestion
digestion
Pepsin A
Chymotrypsin
synthetic peptides
Trypsin
chymotrypsin
Inhibitory Concentration 50
pepsin
Oral Administration
Amino Acid Sequence
trypsin
oral administration

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Novel angiotensin-I-converting enzyme inhibitory peptides derived from recombinant human α(s1)-casein expressed in Escherichia coli. / Kim, Yoo Kyeong; Yoon, Sun; Yu, Dae Yeul; Lönnerdal, Bo; Chung, Bong Hyun.

In: Journal of Dairy Research, Vol. 66, No. 3, 08.1999, p. 431-439.

Research output: Contribution to journalArticle

Kim, Yoo Kyeong ; Yoon, Sun ; Yu, Dae Yeul ; Lönnerdal, Bo ; Chung, Bong Hyun. / Novel angiotensin-I-converting enzyme inhibitory peptides derived from recombinant human α(s1)-casein expressed in Escherichia coli. In: Journal of Dairy Research. 1999 ; Vol. 66, No. 3. pp. 431-439.
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AU - Chung, Bong Hyun

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