Abstract
Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue biodistribution after transplantation. In this study, we assessed the competence of these cells to express, in the absence of differentiation stimuli, markers of mesoderm and ectodermic (neural) cell lineages. Fluorescence microscopy analysis showed a unique pattern of expression of osteogenic, chondrogenic, muscle, and neural markers. The depicted "molecular signature" of these early uncommitted progenitors, in the absence of differentiation stimuli, is consistent with their multipotentiality and plasticity as suggested by several in vitro and in vivo studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 408-414 |
| Number of pages | 7 |
| Journal | Stem Cells and Development |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2005 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Hematology
Cite this
Nonstimulated human uncommitted mesenchymal stem cells express cell markers of mesenchymal and neural lineages. / Minguell, José J.; Fierro, Fernando A; Epuñan, María J.; Erices, Alejandro A.; Sierralta, Walter D.
In: Stem Cells and Development, Vol. 14, No. 4, 08.2005, p. 408-414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nonstimulated human uncommitted mesenchymal stem cells express cell markers of mesenchymal and neural lineages
AU - Minguell, José J.
AU - Fierro, Fernando A
AU - Epuñan, María J.
AU - Erices, Alejandro A.
AU - Sierralta, Walter D.
PY - 2005/8
Y1 - 2005/8
N2 - Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue biodistribution after transplantation. In this study, we assessed the competence of these cells to express, in the absence of differentiation stimuli, markers of mesoderm and ectodermic (neural) cell lineages. Fluorescence microscopy analysis showed a unique pattern of expression of osteogenic, chondrogenic, muscle, and neural markers. The depicted "molecular signature" of these early uncommitted progenitors, in the absence of differentiation stimuli, is consistent with their multipotentiality and plasticity as suggested by several in vitro and in vivo studies.
AB - Ex vivo cultures of human bone marrow-derived mesenchymal stem cells (MSCs) contain subsets of progenitors exhibiting dissimilar properties. One of these subsets comprises uncommitted progenitors displaying distinctive features, such as morphology, a quiescent condition, growth factor production, and restricted tissue biodistribution after transplantation. In this study, we assessed the competence of these cells to express, in the absence of differentiation stimuli, markers of mesoderm and ectodermic (neural) cell lineages. Fluorescence microscopy analysis showed a unique pattern of expression of osteogenic, chondrogenic, muscle, and neural markers. The depicted "molecular signature" of these early uncommitted progenitors, in the absence of differentiation stimuli, is consistent with their multipotentiality and plasticity as suggested by several in vitro and in vivo studies.
UR - http://www.scopus.com/inward/record.url?scp=24144438561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24144438561&partnerID=8YFLogxK
U2 - 10.1089/scd.2005.14.408
DO - 10.1089/scd.2005.14.408
M3 - Article
C2 - 16137230
AN - SCOPUS:24144438561
VL - 14
SP - 408
EP - 414
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 4
ER -