Abstract
Objective. To test the effects of nonsteroidal antiinflammatory drugs (NSAID), naproxen, ibuprofen and diclofenac, and salicylates on normal and interleukin 1 (IL-1) treated human articular chondrocyte metabolism in vitro. Methods. Normal adult human articular chondrocytes were isolated and cultured as primary monolayers; the cells were treated with NSAID and salicylates at low and high plating density for assessing effects on proliferation and matrix synthesis and IL-1 modulated cell metabolism, respectively. Results. Cell proliferation was inhibited by ibuprofen and high doses of salicylates. Glycosaminoglycan (GAG) synthesis was stimulated by ibuprofen at 10 μg/ml but was not changed by any other drugs at similarly low concentrations; at medium to high concentrations, only the salicylates inhibited GAG synthesis. Collagen synthesis was unaffected by any drug at the concentrations tested. IL-1 induced prostaglandin E2 release was completely inhibited by the NSAID and partially inhibited by the salicylates. IL-1 induced IL-6 release was inhibited by ibuprofen and the salicylates whereas IL-1 induced APMA-activated collagenase was only inhibited by the salicylates. Conclusion. Normal human chondrocytes respond differentially to naproxen, ibuprofen, diclofenac, and the salicylates; these observations suggest that each drug may exhibit unique attributes with respect to longterm efficacy on cartilage metabolism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1130-1137 |
| Number of pages | 8 |
| Journal | Journal of Rheumatology |
| Volume | 22 |
| Issue number | 6 |
| State | Published - 1995 |
| Externally published | Yes |
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Keywords
- collagenase
- extracellular matrix
- human articular cartilage
- interleukin 1
- interleukin 6
- nonsteroidal antiinflammatory drugs
ASJC Scopus subject areas
- Immunology
- Rheumatology
Cite this
Nonsteroidal antiinflammatory drugs : Effects on normal and interleukin 1 treated human articular chondrocyte metabolism in vitro. / Smith, R. L.; Kajiyama, G.; Lane, Nancy E.
In: Journal of Rheumatology, Vol. 22, No. 6, 1995, p. 1130-1137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nonsteroidal antiinflammatory drugs
T2 - Effects on normal and interleukin 1 treated human articular chondrocyte metabolism in vitro
AU - Smith, R. L.
AU - Kajiyama, G.
AU - Lane, Nancy E
PY - 1995
Y1 - 1995
N2 - Objective. To test the effects of nonsteroidal antiinflammatory drugs (NSAID), naproxen, ibuprofen and diclofenac, and salicylates on normal and interleukin 1 (IL-1) treated human articular chondrocyte metabolism in vitro. Methods. Normal adult human articular chondrocytes were isolated and cultured as primary monolayers; the cells were treated with NSAID and salicylates at low and high plating density for assessing effects on proliferation and matrix synthesis and IL-1 modulated cell metabolism, respectively. Results. Cell proliferation was inhibited by ibuprofen and high doses of salicylates. Glycosaminoglycan (GAG) synthesis was stimulated by ibuprofen at 10 μg/ml but was not changed by any other drugs at similarly low concentrations; at medium to high concentrations, only the salicylates inhibited GAG synthesis. Collagen synthesis was unaffected by any drug at the concentrations tested. IL-1 induced prostaglandin E2 release was completely inhibited by the NSAID and partially inhibited by the salicylates. IL-1 induced IL-6 release was inhibited by ibuprofen and the salicylates whereas IL-1 induced APMA-activated collagenase was only inhibited by the salicylates. Conclusion. Normal human chondrocytes respond differentially to naproxen, ibuprofen, diclofenac, and the salicylates; these observations suggest that each drug may exhibit unique attributes with respect to longterm efficacy on cartilage metabolism.
AB - Objective. To test the effects of nonsteroidal antiinflammatory drugs (NSAID), naproxen, ibuprofen and diclofenac, and salicylates on normal and interleukin 1 (IL-1) treated human articular chondrocyte metabolism in vitro. Methods. Normal adult human articular chondrocytes were isolated and cultured as primary monolayers; the cells were treated with NSAID and salicylates at low and high plating density for assessing effects on proliferation and matrix synthesis and IL-1 modulated cell metabolism, respectively. Results. Cell proliferation was inhibited by ibuprofen and high doses of salicylates. Glycosaminoglycan (GAG) synthesis was stimulated by ibuprofen at 10 μg/ml but was not changed by any other drugs at similarly low concentrations; at medium to high concentrations, only the salicylates inhibited GAG synthesis. Collagen synthesis was unaffected by any drug at the concentrations tested. IL-1 induced prostaglandin E2 release was completely inhibited by the NSAID and partially inhibited by the salicylates. IL-1 induced IL-6 release was inhibited by ibuprofen and the salicylates whereas IL-1 induced APMA-activated collagenase was only inhibited by the salicylates. Conclusion. Normal human chondrocytes respond differentially to naproxen, ibuprofen, diclofenac, and the salicylates; these observations suggest that each drug may exhibit unique attributes with respect to longterm efficacy on cartilage metabolism.
KW - collagenase
KW - extracellular matrix
KW - human articular cartilage
KW - interleukin 1
KW - interleukin 6
KW - nonsteroidal antiinflammatory drugs
UR - http://www.scopus.com/inward/record.url?scp=0029077580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029077580&partnerID=8YFLogxK
M3 - Article
C2 - 7674242
AN - SCOPUS:0029077580
VL - 22
SP - 1130
EP - 1137
JO - Journal of Rheumatology
JF - Journal of Rheumatology
SN - 0315-162X
IS - 6
ER -