Abstract
Objective. To test the effects of nonsteroidal antiinflammatory drugs (NSAID), naproxen, ibuprofen and diclofenac, and salicylates on normal and interleukin 1 (IL-1) treated human articular chondrocyte metabolism in vitro. Methods. Normal adult human articular chondrocytes were isolated and cultured as primary monolayers; the cells were treated with NSAID and salicylates at low and high plating density for assessing effects on proliferation and matrix synthesis and IL-1 modulated cell metabolism, respectively. Results. Cell proliferation was inhibited by ibuprofen and high doses of salicylates. Glycosaminoglycan (GAG) synthesis was stimulated by ibuprofen at 10 μg/ml but was not changed by any other drugs at similarly low concentrations; at medium to high concentrations, only the salicylates inhibited GAG synthesis. Collagen synthesis was unaffected by any drug at the concentrations tested. IL-1 induced prostaglandin E2 release was completely inhibited by the NSAID and partially inhibited by the salicylates. IL-1 induced IL-6 release was inhibited by ibuprofen and the salicylates whereas IL-1 induced APMA-activated collagenase was only inhibited by the salicylates. Conclusion. Normal human chondrocytes respond differentially to naproxen, ibuprofen, diclofenac, and the salicylates; these observations suggest that each drug may exhibit unique attributes with respect to longterm efficacy on cartilage metabolism.
Original language | English (US) |
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Pages (from-to) | 1130-1137 |
Number of pages | 8 |
Journal | Journal of Rheumatology |
Volume | 22 |
Issue number | 6 |
State | Published - 1995 |
Externally published | Yes |
Keywords
- collagenase
- extracellular matrix
- human articular cartilage
- interleukin 1
- interleukin 6
- nonsteroidal antiinflammatory drugs
ASJC Scopus subject areas
- Immunology
- Rheumatology