Nonshivering thermogenesis and brown fat in spontaneously hypertensive rats.

S. Hayashi, S. J. Wickler, S. Gray, Barbara A Horwitz

Research output: Contribution to journalArticle

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Abstract

Oxygen consumption was measured before and during infusion of the catecholamine isoproterenol in age-matched spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) normotensive rats. Mass-independent rates of oxygen consumption of anesthetized 7-week-old rats were similar in the WKY and SHR rats (11.08 +/- 0.74 and 11.33 +/- 0.82 ml O2 min-1 kg-.67, respectively). Catecholamine infusion elicited increased total oxygen consumption in both WKY and SHR animals (15.0 +/- 1.0 and 14.9 +/- 1.2 ml O2 min-1 kg-.67, respectively), and the magnitude of these increases did not significantly differ. To assess whether there were changes in the metabolic state of brown adipose tissue, the major site of catecholamine-induced thermogenesis in rats, enzymes whose activity is proportional to aerobic capacity were assayed in vitro. In both the interscapular and cervical brown fat depots, maximal citrate synthase and maximal HOAD (beta-hydroxyacyl-CoA dehydrogenase) activities were similar in SHR and WKY rats. There were also no significant differences in brown fat protein content, suggesting no differential growth of this tissue in the two rat strains. Our results indicate that the nonshivering thermogenic capacity of the hypertensive SHR rats does not differ from that of the normotensive WKY animals.

Original languageEnglish (US)
Pages (from-to)435-439
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume188
Issue number4
StatePublished - Sep 1988

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Brown Adipose Tissue
Thermogenesis
Inbred SHR Rats
Oxygen Consumption
Catecholamines
Rats
Inbred WKY Rats
Fats
3-Hydroxyacyl CoA Dehydrogenases
Citrate (si)-Synthase
Coenzyme A
Isoproterenol
Oxygen
Animals
Tissue
Enzymes
Growth
Enzyme activity
Proteins
Oxidoreductases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nonshivering thermogenesis and brown fat in spontaneously hypertensive rats. / Hayashi, S.; Wickler, S. J.; Gray, S.; Horwitz, Barbara A.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 188, No. 4, 09.1988, p. 435-439.

Research output: Contribution to journalArticle

Hayashi, S. ; Wickler, S. J. ; Gray, S. ; Horwitz, Barbara A. / Nonshivering thermogenesis and brown fat in spontaneously hypertensive rats. In: Proceedings of the Society for Experimental Biology and Medicine. 1988 ; Vol. 188, No. 4. pp. 435-439.
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