Nonsaturation of AMPA and NMDA receptors at hippocampal synapses

A Kimberley Usrey, Charles F. Stevens

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

An important issue in synaptic physiology is the extent to which postsynaptic receptors are saturated by the neurotransmitter released from a single synaptic vesicle. Although the bulk of evidence supports receptor saturation, recent studies have started to reveal that α-amino-3-hydroxy-5- methyl-4-isoxazolepropionate (AMPA) and N-methyl-D-aspartate (NMDA) receptors may not be saturated by a single vesicle of glutamate. Here, we address this question through a study of putative single synapses, made by hippocampal neurons in culture, that are identified by FM1-43 staining. An analysis of the sources of variability in the amplitudes of miniature excitatory postsynaptic currents at single synapses reveals that this variability must arise presynaptically, from variations in the quantity of agonist released. Thus, glutamate receptors at hippocampal synapses are not generally saturated by quantal release.

Original languageEnglish (US)
Pages (from-to)6173-6178
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number11
DOIs
StatePublished - May 23 2000

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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
N-Methyl-D-Aspartate Receptors
Synapses
Neurotransmitter Receptor
Synaptic Vesicles
Excitatory Postsynaptic Potentials
Glutamate Receptors
Glutamic Acid
Staining and Labeling
Neurons

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Nonsaturation of AMPA and NMDA receptors at hippocampal synapses. / Usrey, A Kimberley; Stevens, Charles F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 11, 23.05.2000, p. 6173-6178.

Research output: Contribution to journalArticle

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