Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles

Diego Rativa; Anderson S L Gomes; Sebastian Wachsmann-Hogiu; Daniel L. Farkas; Renato E. De Araujo

doi:10.1007/s10895-008-0366-6

Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles

Diego Rativa, Anderson S L Gomes, Sebastian Wachsmann-Hogiu, Daniel L. Farkas, Renato E. De Araujo

Pathology and Laboratory Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.

Original language	English (US)
Pages (from-to)	1151-1155
Number of pages	5
Journal	Journal of Fluorescence
Volume	18
Issue number	6
DOIs	https://doi.org/10.1007/s10895-008-0366-6
State	Published - Nov 2008

Keywords

Autofluorescence
Metallic nanoparticles
Nonlinear excitation
Tryptophan

ASJC Scopus subject areas

Spectroscopy
Biochemistry
Clinical Biochemistry

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title = "Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles",

abstract = "We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.",

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T1 - Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles

AU - Rativa, Diego

AU - Gomes, Anderson S L

AU - Wachsmann-Hogiu, Sebastian

AU - Farkas, Daniel L.

AU - De Araujo, Renato E.

PY - 2008/11

Y1 - 2008/11

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AB - We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.

KW - Autofluorescence

KW - Metallic nanoparticles

KW - Nonlinear excitation

KW - Tryptophan

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