Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles

Diego Rativa, Anderson S L Gomes, Sebastian Wachsmann-Hogiu, Daniel L. Farkas, Renato E. De Araujo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.

Original languageEnglish (US)
Pages (from-to)1151-1155
Number of pages5
JournalJournal of Fluorescence
Volume18
Issue number6
DOIs
StatePublished - Nov 2008

Fingerprint

Photons
Silver
Tryptophan
Nanoparticles
Fluorescence
water
experiment
Colloids
Metal Nanoparticles
Biomolecules
Light
Amino Acids
Quenching
Water
Infrared radiation
Experiments

Keywords

  • Autofluorescence
  • Metallic nanoparticles
  • Nonlinear excitation
  • Tryptophan

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry
  • Clinical Biochemistry

Cite this

Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles. / Rativa, Diego; Gomes, Anderson S L; Wachsmann-Hogiu, Sebastian; Farkas, Daniel L.; De Araujo, Renato E.

In: Journal of Fluorescence, Vol. 18, No. 6, 11.2008, p. 1151-1155.

Research output: Contribution to journalArticle

Rativa, Diego ; Gomes, Anderson S L ; Wachsmann-Hogiu, Sebastian ; Farkas, Daniel L. ; De Araujo, Renato E. / Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles. In: Journal of Fluorescence. 2008 ; Vol. 18, No. 6. pp. 1151-1155.
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