Abstract
We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1151-1155 |
| Number of pages | 5 |
| Journal | Journal of Fluorescence |
| Volume | 18 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 2008 |
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Keywords
- Autofluorescence
- Metallic nanoparticles
- Nonlinear excitation
- Tryptophan
ASJC Scopus subject areas
- Spectroscopy
- Biochemistry
- Clinical Biochemistry
Cite this
Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles. / Rativa, Diego; Gomes, Anderson S L; Wachsmann-Hogiu, Sebastian; Farkas, Daniel L.; De Araujo, Renato E.
In: Journal of Fluorescence, Vol. 18, No. 6, 11.2008, p. 1151-1155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nonlinear excitation of tryptophan emission enhanced by silver nanoparticles
AU - Rativa, Diego
AU - Gomes, Anderson S L
AU - Wachsmann-Hogiu, Sebastian
AU - Farkas, Daniel L.
AU - De Araujo, Renato E.
PY - 2008/11
Y1 - 2008/11
N2 - We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.
AB - We measured and analyzed the behavior of the fluorescence of tryptophan water solutions with and without silver nanoparticles, excited by one, two and three photon processes. Two different colloids with silver nanoparticles with distinct diameters (0.65 nm and 9 nm) were used in the experiments. Fluorescence quenching was observed with one and two photon excitation. However, upon three-photon excitation, significant fluorescence enhancement was observed in the colloid. In this case excitation of the amino acid is assisted by the nonlinear absorption of infrared light by the silver nanoparticles. In this paper we are proposing a new way to explore metallic nanoparticles to enhance autofluorescence of biomolecules.
KW - Autofluorescence
KW - Metallic nanoparticles
KW - Nonlinear excitation
KW - Tryptophan
UR - http://www.scopus.com/inward/record.url?scp=56749163821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56749163821&partnerID=8YFLogxK
U2 - 10.1007/s10895-008-0366-6
DO - 10.1007/s10895-008-0366-6
M3 - Article
C2 - 18379860
AN - SCOPUS:56749163821
VL - 18
SP - 1151
EP - 1155
JO - Journal of Fluorescence
JF - Journal of Fluorescence
SN - 1053-0509
IS - 6
ER -