Noninvasive measurement of myocardial activity concentrations and perfusion defect sizes in rats with a new small-animal positron emission tomograph

Takashi Kudo, Kazuki Fukuchi, Alexander J. Annala, Arion F. Chatziioannou, Vivekanand Allada, Magnus Dahlbom, Yuan Chuan Tai, Masayuki Inubushi, Sung Cheng Huang, Simon R Cherry, Michael E. Phelps, Heinrich R. Schelbert

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Background - We explored the feasibility of measuring regional tracer activity concentrations and flow defects in myocardium of rats with a high spatial resolution small-animal PET system (microPET). Methods and Results - Myocardial images were obtained after intravenous 18F-fluorodeoxyglucose (18FDG) in 11 normal rats (group 1) and assembled into polar maps. Regional 18F activity concentrations were measured in 9 regions of interest and compared with tissue activity concentrations measured by well counting. In another 9 rats (group 2), myocardial perfusion images were acquired with 13N-ammonia at baseline and during coronary occlusion. On the polar maps recorded during coronary occlusion, the size of perfusion defects was measured as the myocardium with <50% of maximum activity and expressed as percent total myocardium and was correlated with the area at risk defined by postmortem staining. The diagnostic quality of 18FDG and 13N-ammonia microPET images was good to excellent; the images were easily assembled into polar maps. In group 1, regional 18F concentrations by microPET and postmortem were correlated linearly (r=0.99; P<0.01 for average and r=0.97; P<0.01 for regional concentrations). In group 2, perfusion defect sizes by microPET and postmortem were correlated linearly (P<0.01; r=0.93). Conclusions - The findings indicate the feasibility of noninvasive studies of the myocardium in rats with a dedicated small-animal PET-imaging device.

Original languageEnglish (US)
Pages (from-to)118-123
Number of pages6
JournalCirculation
Volume106
Issue number1
DOIs
StatePublished - Jul 1 2002

Keywords

  • Imaging
  • Myocardium
  • Perfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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