Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography

Shau Poh Chong, Conrad W. Merkle, Dylan F. Cooke, Tingwei Zhang, Harsha Radhakrishnan, Leah Krubitzer, Vivek Srinivasan

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A spectral/Fourier domain optical coherence tomography (OCT) intravital microscope using a supercontinuum light source at 1.7 μm was developed to study subcortical structures noninvasively in the living mouse brain. The benefits of 1.7 μm for deep tissue brain imaging are demonstrated by quantitatively comparing OCT signal attenuation characteristics of cortical tissue across visible and near-infrared wavelengths. Imaging of hippocampal tissue architecture and white matter microvasculature are demonstrated in vivo through thinned-skull, glass coverslip-reinforced cranial windows in mice. Applications of this novel platform include monitoring disease progression and pathophysiology in rodent models of Alzheimer's disease and subcortical dementias, including vascular dementia.

Original languageEnglish (US)
Pages (from-to)4911-4914
Number of pages4
JournalOptics Letters
Volume40
Issue number21
DOIs
StatePublished - Nov 1 2015

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brain
mice
tomography
rodents
skull
progressions
light sources
platforms
attenuation
microscopes
glass
wavelengths

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Chong, S. P., Merkle, C. W., Cooke, D. F., Zhang, T., Radhakrishnan, H., Krubitzer, L., & Srinivasan, V. (2015). Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography. Optics Letters, 40(21), 4911-4914. https://doi.org/10.1364/OL.40.004911

Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography. / Chong, Shau Poh; Merkle, Conrad W.; Cooke, Dylan F.; Zhang, Tingwei; Radhakrishnan, Harsha; Krubitzer, Leah; Srinivasan, Vivek.

In: Optics Letters, Vol. 40, No. 21, 01.11.2015, p. 4911-4914.

Research output: Contribution to journalArticle

Chong, SP, Merkle, CW, Cooke, DF, Zhang, T, Radhakrishnan, H, Krubitzer, L & Srinivasan, V 2015, 'Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography', Optics Letters, vol. 40, no. 21, pp. 4911-4914. https://doi.org/10.1364/OL.40.004911
Chong SP, Merkle CW, Cooke DF, Zhang T, Radhakrishnan H, Krubitzer L et al. Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography. Optics Letters. 2015 Nov 1;40(21):4911-4914. https://doi.org/10.1364/OL.40.004911
Chong, Shau Poh ; Merkle, Conrad W. ; Cooke, Dylan F. ; Zhang, Tingwei ; Radhakrishnan, Harsha ; Krubitzer, Leah ; Srinivasan, Vivek. / Noninvasive, in vivo imaging of subcortical mouse brain regions with 1.7 μm optical coherence tomography. In: Optics Letters. 2015 ; Vol. 40, No. 21. pp. 4911-4914.
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