Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy

Peter Ashjian, Amir Elbarbary, Patricia Zuk, Daniel A. DeUgarte, Prosper Benhaim, Laura Marcu, Marc H. Hedrick

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.

Original languageEnglish (US)
Pages (from-to)411-420
Number of pages10
JournalTissue Engineering
Volume10
Issue number3-4
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Fluorescence Spectrometry
Fluorescence spectroscopy
Lasers
Collagen
Tissue
Extracellular Matrix
Collagen Type III
Collagen Type I
Fluorescent Antibody Technique
Adipose Tissue
Spectrum Analysis
Stem Cells
Transplantation
Stem cells
Western Blotting
Monitoring

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

Cite this

Ashjian, P., Elbarbary, A., Zuk, P., DeUgarte, D. A., Benhaim, P., Marcu, L., & Hedrick, M. H. (2004). Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy. Tissue Engineering, 10(3-4), 411-420. https://doi.org/10.1089/107632704323061771

Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy. / Ashjian, Peter; Elbarbary, Amir; Zuk, Patricia; DeUgarte, Daniel A.; Benhaim, Prosper; Marcu, Laura; Hedrick, Marc H.

In: Tissue Engineering, Vol. 10, No. 3-4, 03.2004, p. 411-420.

Research output: Contribution to journalArticle

Ashjian, P, Elbarbary, A, Zuk, P, DeUgarte, DA, Benhaim, P, Marcu, L & Hedrick, MH 2004, 'Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy', Tissue Engineering, vol. 10, no. 3-4, pp. 411-420. https://doi.org/10.1089/107632704323061771
Ashjian, Peter ; Elbarbary, Amir ; Zuk, Patricia ; DeUgarte, Daniel A. ; Benhaim, Prosper ; Marcu, Laura ; Hedrick, Marc H. / Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy. In: Tissue Engineering. 2004 ; Vol. 10, No. 3-4. pp. 411-420.
@article{85c837cf2bc9420a8f89391932c6ab74,
title = "Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy",
abstract = "The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.",
author = "Peter Ashjian and Amir Elbarbary and Patricia Zuk and DeUgarte, {Daniel A.} and Prosper Benhaim and Laura Marcu and Hedrick, {Marc H.}",
year = "2004",
month = "3",
doi = "10.1089/107632704323061771",
language = "English (US)",
volume = "10",
pages = "411--420",
journal = "Tissue Engineering",
issn = "1076-3279",
publisher = "Mary Ann Liebert Inc.",
number = "3-4",

}

TY - JOUR

T1 - Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy

AU - Ashjian, Peter

AU - Elbarbary, Amir

AU - Zuk, Patricia

AU - DeUgarte, Daniel A.

AU - Benhaim, Prosper

AU - Marcu, Laura

AU - Hedrick, Marc H.

PY - 2004/3

Y1 - 2004/3

N2 - The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.

AB - The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.

UR - http://www.scopus.com/inward/record.url?scp=2342513440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342513440&partnerID=8YFLogxK

U2 - 10.1089/107632704323061771

DO - 10.1089/107632704323061771

M3 - Article

C2 - 15165458

AN - SCOPUS:2342513440

VL - 10

SP - 411

EP - 420

JO - Tissue Engineering

JF - Tissue Engineering

SN - 1076-3279

IS - 3-4

ER -