Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy

Peter Ashjian, Amir Elbarbary, Patricia Zuk, Daniel A. DeUgarte, Prosper Benhaim, Laura Marcu, Marc H. Hedrick

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.

Original languageEnglish (US)
Pages (from-to)411-420
Number of pages10
JournalTissue Engineering
Volume10
Issue number3-4
DOIs
StatePublished - Mar 2004
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology

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    Ashjian, P., Elbarbary, A., Zuk, P., DeUgarte, D. A., Benhaim, P., Marcu, L., & Hedrick, M. H. (2004). Noninvasive in Situ Evaluation of Osteogenic Differentiation by Time-Resolved Laser-Induced Fluorescence Spectroscopy. Tissue Engineering, 10(3-4), 411-420. https://doi.org/10.1089/107632704323061771