Nondestructive fluorescence lifetime imaging and time-resolved fluorescence spectroscopy detect cartilage matrix depletion and correlate with mechanical properties

A. K. Haudenschild, B. E. Sherlock, X. Zhou, J. C. Hu, Jonathan K Leach, Laura Marcu, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Tissue engineers utilize a battery of expensive, time-consuming and destructive techniques to assess the composition and function of engineered tissues. A nondestructive solution to monitor tissue maturation would reduce costs and accelerate product development. As a first step toward this goal, two nondestructive, label-free optical techniques, namely multispectral fluorescent lifetime imaging (FLIm) and time-resolved fluorescence spectroscopy (TRFS), were investigated for their potential in evaluating the biochemical and mechanical properties of articular cartilage. Enzymatic treatments were utilized to selectively deplete cartilage of either collagen or proteoglycan, to produce a range of matrix compositions. Samples were assessed for their optical properties using a fiber-coupled optical system combining FLIm and TRFS, their biochemical and mechanical properties and by histological staining. Single and multivariable correlations were performed to evaluate relationships among these properties. FLIm-and TRFS-derived measurements are sensitive to changes in cartilage matrix and correlate with mechanical and biochemical assays. Mean fluorescence lifetime values extracted from FLIm images (375-410 nm spectral band) showed strong, specific correlations with collagen content (R2 = 0.79, p < 0.001) and tensile properties (R2 = 0.45, p = 0.02). TRFS lifetime measurements centered at 520 nm (with a 5 nm bandwidth) possessed strong, specific correlations with proteoglycan content (R2 = 0.59, p = 0.001) and compressive properties (R2 = 0.71, p < 0.001). Nondestructive optical assessment of articular cartilage, using a combination of FLIm-and TRFS-derived parameters, provided a quantitative method for determining tissue biochemical composition and mechanical function. These tools hold great potential for research, industrial and clinical settings.

Original languageEnglish (US)
Pages (from-to)30-43
Number of pages14
JournalEuropean Cells and Materials
Volume36
DOIs
StatePublished - Jul 1 2018

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Optical Imaging
Fluorescence Spectrometry
Fluorescence spectroscopy
Cartilage
Fluorescence
Imaging techniques
Mechanical properties
Tissue
Proteoglycans
Collagen
Articular Cartilage
Chemical analysis
Industrial research
Optical fiber coupling
Optical Devices
Tensile properties
Product development
Optical systems
Labels
Assays

Keywords

  • Biomechanics
  • Cartilage
  • Cartilage repair and regeneration
  • Collagens
  • Extracellular matrix
  • Imaging
  • Proteoglycans
  • Regenerative medicine
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Cell Biology

Cite this

Nondestructive fluorescence lifetime imaging and time-resolved fluorescence spectroscopy detect cartilage matrix depletion and correlate with mechanical properties. / Haudenschild, A. K.; Sherlock, B. E.; Zhou, X.; Hu, J. C.; Leach, Jonathan K; Marcu, Laura; Athanasiou, Kyriacos A.

In: European Cells and Materials, Vol. 36, 01.07.2018, p. 30-43.

Research output: Contribution to journalArticle

Haudenschild, A. K. ; Sherlock, B. E. ; Zhou, X. ; Hu, J. C. ; Leach, Jonathan K ; Marcu, Laura ; Athanasiou, Kyriacos A. / Nondestructive fluorescence lifetime imaging and time-resolved fluorescence spectroscopy detect cartilage matrix depletion and correlate with mechanical properties. In: European Cells and Materials. 2018 ; Vol. 36. pp. 30-43.
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abstract = "Tissue engineers utilize a battery of expensive, time-consuming and destructive techniques to assess the composition and function of engineered tissues. A nondestructive solution to monitor tissue maturation would reduce costs and accelerate product development. As a first step toward this goal, two nondestructive, label-free optical techniques, namely multispectral fluorescent lifetime imaging (FLIm) and time-resolved fluorescence spectroscopy (TRFS), were investigated for their potential in evaluating the biochemical and mechanical properties of articular cartilage. Enzymatic treatments were utilized to selectively deplete cartilage of either collagen or proteoglycan, to produce a range of matrix compositions. Samples were assessed for their optical properties using a fiber-coupled optical system combining FLIm and TRFS, their biochemical and mechanical properties and by histological staining. Single and multivariable correlations were performed to evaluate relationships among these properties. FLIm-and TRFS-derived measurements are sensitive to changes in cartilage matrix and correlate with mechanical and biochemical assays. Mean fluorescence lifetime values extracted from FLIm images (375-410 nm spectral band) showed strong, specific correlations with collagen content (R2 = 0.79, p < 0.001) and tensile properties (R2 = 0.45, p = 0.02). TRFS lifetime measurements centered at 520 nm (with a 5 nm bandwidth) possessed strong, specific correlations with proteoglycan content (R2 = 0.59, p = 0.001) and compressive properties (R2 = 0.71, p < 0.001). Nondestructive optical assessment of articular cartilage, using a combination of FLIm-and TRFS-derived parameters, provided a quantitative method for determining tissue biochemical composition and mechanical function. These tools hold great potential for research, industrial and clinical settings.",
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AU - Athanasiou, Kyriacos A.

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