Noncytotoxic Clostridium perfringens enterotoxin (CPE) variants localize CPE intestinal binding and demonstrate a relationship between CPE-induced cytotoxicity and enterotoxicity

James G. Smedley, Juliann Saputo, Jacquelyn C. Parker, Mariano E. Fernandez-Miyakawa, Susan L. Robertson, Bruce A. McClane, Francisco A Uzal

Research output: Contribution to journalArticle

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Abstract

Clostridium perfringens enterotoxin (CPE) causes the symptoms of a very common food poisoning. To assess whether CPE-induced cytotoxicity is necessary for enterotoxicity, a rabbit ileal loop model was used to compare the in vivo effects of native CPE or recombinant CPE (rCPE), both of which are cytotoxic, with those of the noncytotoxic rCPE variants rCPE D48A and rCPE 168-319. Both CPE and rCPE elicited significant fluid accumulation in rabbit ileal loops, along with severe mucosal damage that starts at villus tips and then progressively affects the entire villus, including necrosis of epithelium and lamina propria, villus blunting and fusion, and transmural edema and hemorrhage. Similar treatment of ileal loops with either of the noncytotoxic rCPE variants produced no visible histologic damage or fluid transport changes. Immunohistochemistry revealed strong CPE or rCPE168-319 binding to villus tips, which correlated with the abundant presence of claudin-4, a known CPE receptor, in this villus region. These results support (i) cytotoxicity being necessary for CPE-induced enterotoxicity, (ii) the CPE sensitivity of villus tips being at least partially attributable to the abundant presence of receptors in this villus region, and (iii) claudin-4 being an important intestinal receptor for CPE. Finally, rCPE168-319 was able to partially inhibit CPE-induced histologic damage, suggesting that noncytotoxic rCPE variants might be useful for protecting against some intestinal effects of CPE.

Original languageEnglish (US)
Pages (from-to)3793-3800
Number of pages8
JournalInfection and Immunity
Volume76
Issue number8
DOIs
StatePublished - Aug 2008

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Claudin-4
Clostridium enterotoxin
Rabbits
Foodborne Diseases
Edema
Mucous Membrane
Necrosis
Epithelium
Immunohistochemistry
Hemorrhage
Clostridium perfringens enterotoxin receptor
Therapeutics

ASJC Scopus subject areas

  • Immunology

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Noncytotoxic Clostridium perfringens enterotoxin (CPE) variants localize CPE intestinal binding and demonstrate a relationship between CPE-induced cytotoxicity and enterotoxicity. / Smedley, James G.; Saputo, Juliann; Parker, Jacquelyn C.; Fernandez-Miyakawa, Mariano E.; Robertson, Susan L.; McClane, Bruce A.; Uzal, Francisco A.

In: Infection and Immunity, Vol. 76, No. 8, 08.2008, p. 3793-3800.

Research output: Contribution to journalArticle

Smedley, James G. ; Saputo, Juliann ; Parker, Jacquelyn C. ; Fernandez-Miyakawa, Mariano E. ; Robertson, Susan L. ; McClane, Bruce A. ; Uzal, Francisco A. / Noncytotoxic Clostridium perfringens enterotoxin (CPE) variants localize CPE intestinal binding and demonstrate a relationship between CPE-induced cytotoxicity and enterotoxicity. In: Infection and Immunity. 2008 ; Vol. 76, No. 8. pp. 3793-3800.
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