Non-viral gene delivery regulated by stiffness of cell adhesion substrates

Hyun Joon Kong, Jodi Liu, Kathryn Riddle, Takuya Matsumoto, Jonathan K Leach, David J. Mooney

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

The rigidity of the cell adhesion substrate on the level of gene transfer and expression, was investigated. Gene-transfer efficiency was investigated using a fluorescent resonance energy transfer (FRET) technique, as FRET techniques have been extensively used to evaluate specific intermolecular interactions at the nanometre scale. The mechanical properties of the substrate to which cell adheres have been found to mediate many aspects of cell function including proliferation, migration and differentiations. The study indicated the importance of material mechanics in regulating gene transfer and expression, and also enabled to understand the gene transfer processes in different tissues.

Original languageEnglish (US)
Pages (from-to)460-464
Number of pages5
JournalNature Materials
Volume4
Issue number6
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Gene transfer
Cell adhesion
genes
stiffness
delivery
adhesion
Genes
Stiffness
Substrates
cells
Gene expression
Energy transfer
energy transfer
rigidity
Rigidity
Mechanics
mechanical properties
Tissue
Mechanical properties
interactions

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kong, H. J., Liu, J., Riddle, K., Matsumoto, T., Leach, J. K., & Mooney, D. J. (2005). Non-viral gene delivery regulated by stiffness of cell adhesion substrates. Nature Materials, 4(6), 460-464. https://doi.org/10.1038/nmat1392

Non-viral gene delivery regulated by stiffness of cell adhesion substrates. / Kong, Hyun Joon; Liu, Jodi; Riddle, Kathryn; Matsumoto, Takuya; Leach, Jonathan K; Mooney, David J.

In: Nature Materials, Vol. 4, No. 6, 06.2005, p. 460-464.

Research output: Contribution to journalArticle

Kong, HJ, Liu, J, Riddle, K, Matsumoto, T, Leach, JK & Mooney, DJ 2005, 'Non-viral gene delivery regulated by stiffness of cell adhesion substrates', Nature Materials, vol. 4, no. 6, pp. 460-464. https://doi.org/10.1038/nmat1392
Kong HJ, Liu J, Riddle K, Matsumoto T, Leach JK, Mooney DJ. Non-viral gene delivery regulated by stiffness of cell adhesion substrates. Nature Materials. 2005 Jun;4(6):460-464. https://doi.org/10.1038/nmat1392
Kong, Hyun Joon ; Liu, Jodi ; Riddle, Kathryn ; Matsumoto, Takuya ; Leach, Jonathan K ; Mooney, David J. / Non-viral gene delivery regulated by stiffness of cell adhesion substrates. In: Nature Materials. 2005 ; Vol. 4, No. 6. pp. 460-464.
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