Non-toxic thermotropic liquid crystals for use with mammalian cells

Yan Yeung Luk, Sean F. Campbell, Nicholas L. Abbott, Christopher J Murphy

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

This paper reports the results of a study that aimed to identify thermotropic liquid crystals that are not toxic to mammalian cells. Mesogenic compounds were mixed to create eight liquid crystalline phases, each with a unique set of functional groups. We investigated the toxicity of each liquid crystalline phase using two mammalian cell lines - 3T3 fibroblast and SV-40 transformed human corneal epithelial (HCEC) cells. Using dual fluorescent staining assays based on calcein acetoxymethylester (Calcein-AM) and ethidium homodimer, we measured correspondingly the number of viable and dead cells following immersion of the cells in the liquid crystals. It was found that most of the liquid crystals, such as commonly used 5CB and E7, caused cell death after contact with cells for four hours. However, we identified a class of liquid crystals containing fluorophenyl groups that possess minimal or no toxicity (as indicated by results of assays based on Calcein-AM and ethidium homodimer) to cells. Following immersion in fluorophenyl liquid crystals for four hours, the cells were observed to proliferate in culture medium at rates similar to control cells exposed to phosphate-buffered saline (PBS) for four hours. In contrast, treatment of cells for 24 hours with either PBS or liquid crystals (media containing no nutrients and growth factors) resulted in cell death.

Original languageEnglish (US)
Pages (from-to)611-621
Number of pages11
JournalLiquid Crystals
Volume31
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

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Thermotropic liquid crystals
Liquid Crystals
Liquid crystals
liquid crystals
Cells
cells
Cell death
Toxicity
Assays
Phosphates
death
toxicity
Crystalline materials
submerging
phosphates
Poisons
Liquids
Fibroblasts
Functional groups
Nutrients

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

Non-toxic thermotropic liquid crystals for use with mammalian cells. / Luk, Yan Yeung; Campbell, Sean F.; Abbott, Nicholas L.; Murphy, Christopher J.

In: Liquid Crystals, Vol. 31, No. 5, 05.2004, p. 611-621.

Research output: Contribution to journalArticle

Luk, Yan Yeung ; Campbell, Sean F. ; Abbott, Nicholas L. ; Murphy, Christopher J. / Non-toxic thermotropic liquid crystals for use with mammalian cells. In: Liquid Crystals. 2004 ; Vol. 31, No. 5. pp. 611-621.
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