Abstract
Five patients with heterozygous familial hypercholesterolaemia (EH), one patient with nonfamilial hypercholesterolaemia, and four healthy normals were treated with plasma exchange (PE). In order to achieve a non-steady-state situation with low plasma cholesterol levels, PE was performed twice in each subject at an interval of 1-3 days. At each time, 1500 ml of plasma were removed and replaced with colloids and albumin. Lipoprotein analyses were performed daily in all subjects for a period of 14 days following PE. Kinetic calculation of LDL cholesterol data using a two-compartment open model was performed, and rate constants, synthetic rate and apparent clearance of LDL were determined. A lower clearance and a tendency towards a higher synthesis of LDL were observed in the FH patients. In four of the subjects, LDL kinetics was also studied with the conventional 125I-LDL turnover technique. The synthetic rates of LDL were compared using data derived from PE and 125I-LDL kinetics. The two techniques showed a good correlation (r = +0.93) although synthetic rates in general were higher when calculated from the 125I-LDL procedure (3.98 vs. 2.64 mmol d-1 for LDL cholesterol synthesis). The results indicate that non-steady-state kinetics of LDL, as measured using the PE technique, is a useful method for analysis of LDL metabolism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 746-752 |
| Number of pages | 7 |
| Journal | European Journal of Clinical Investigation |
| Volume | 23 |
| Issue number | 11 |
| State | Published - 1993 |
| Externally published | Yes |
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Keywords
- cholesterol
- fractional catabolic rate
- LDL apheresis
- metabolic regulation
ASJC Scopus subject areas
- Medicine(all)
Cite this
Non-steady-state kinetics of low density lipoproteins in man : Studies after plasma exchange in healthy subjects and patients with familial hypercholesterolaemia. / Eriksson, M.; Berglund, Lars; Gabrielsson, J.; Lantz, B.; Angelin, B.
In: European Journal of Clinical Investigation, Vol. 23, No. 11, 1993, p. 746-752.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Non-steady-state kinetics of low density lipoproteins in man
T2 - Studies after plasma exchange in healthy subjects and patients with familial hypercholesterolaemia
AU - Eriksson, M.
AU - Berglund, Lars
AU - Gabrielsson, J.
AU - Lantz, B.
AU - Angelin, B.
PY - 1993
Y1 - 1993
N2 - Five patients with heterozygous familial hypercholesterolaemia (EH), one patient with nonfamilial hypercholesterolaemia, and four healthy normals were treated with plasma exchange (PE). In order to achieve a non-steady-state situation with low plasma cholesterol levels, PE was performed twice in each subject at an interval of 1-3 days. At each time, 1500 ml of plasma were removed and replaced with colloids and albumin. Lipoprotein analyses were performed daily in all subjects for a period of 14 days following PE. Kinetic calculation of LDL cholesterol data using a two-compartment open model was performed, and rate constants, synthetic rate and apparent clearance of LDL were determined. A lower clearance and a tendency towards a higher synthesis of LDL were observed in the FH patients. In four of the subjects, LDL kinetics was also studied with the conventional 125I-LDL turnover technique. The synthetic rates of LDL were compared using data derived from PE and 125I-LDL kinetics. The two techniques showed a good correlation (r = +0.93) although synthetic rates in general were higher when calculated from the 125I-LDL procedure (3.98 vs. 2.64 mmol d-1 for LDL cholesterol synthesis). The results indicate that non-steady-state kinetics of LDL, as measured using the PE technique, is a useful method for analysis of LDL metabolism.
AB - Five patients with heterozygous familial hypercholesterolaemia (EH), one patient with nonfamilial hypercholesterolaemia, and four healthy normals were treated with plasma exchange (PE). In order to achieve a non-steady-state situation with low plasma cholesterol levels, PE was performed twice in each subject at an interval of 1-3 days. At each time, 1500 ml of plasma were removed and replaced with colloids and albumin. Lipoprotein analyses were performed daily in all subjects for a period of 14 days following PE. Kinetic calculation of LDL cholesterol data using a two-compartment open model was performed, and rate constants, synthetic rate and apparent clearance of LDL were determined. A lower clearance and a tendency towards a higher synthesis of LDL were observed in the FH patients. In four of the subjects, LDL kinetics was also studied with the conventional 125I-LDL turnover technique. The synthetic rates of LDL were compared using data derived from PE and 125I-LDL kinetics. The two techniques showed a good correlation (r = +0.93) although synthetic rates in general were higher when calculated from the 125I-LDL procedure (3.98 vs. 2.64 mmol d-1 for LDL cholesterol synthesis). The results indicate that non-steady-state kinetics of LDL, as measured using the PE technique, is a useful method for analysis of LDL metabolism.
KW - cholesterol
KW - fractional catabolic rate
KW - LDL apheresis
KW - metabolic regulation
UR - http://www.scopus.com/inward/record.url?scp=0027521281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027521281&partnerID=8YFLogxK
M3 - Article
C2 - 8307094
AN - SCOPUS:0027521281
VL - 23
SP - 746
EP - 752
JO - European Journal of Clinical Investigation
JF - European Journal of Clinical Investigation
SN - 0014-2972
IS - 11
ER -