Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure

Chengji J. Zhou; Yu Ji; Kurt Reynolds; Moira McMahon; Michael A. Garland; Shuwen Zhang; Bo Sun; Ran Gu; Mohammad Islam; Yue Liu; Tianyu Zhao; Grace Hsu; Janet Iwasa

doi:10.1016/j.bbrc.2020.03.138

Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure

Chengji J. Zhou, Yu Ji, Kurt Reynolds, Moira McMahon, Michael A. Garland, Shuwen Zhang, Bo Sun, Ran Gu, Mohammad Islam, Yue Liu, Tianyu Zhao, Grace Hsu, Janet Iwasa

Biochemistry and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The mechanisms underlying mammalian neural tube closure remain poorly understood. We report a unique cellular process involving multicellular rosette formation, convergent cellular protrusions, and F-actin cable network of the non-neural surface ectodermal cells encircling the closure site of the posterior neuropore, which are demonstrated by scanning electron microscopy and genetic fate mapping analyses during mouse spinal neurulation. These unique cellular structures are severely disrupted in the surface ectodermal transcription factor Grhl3 mutants that exhibit fully penetrant spina bifida. We propose a novel model of mammalian neural tube closure driven by surface ectodermal dynamics, which is computationally visualized.

Original language	English (US)
Journal	Biochemical and Biophysical Research Communications
DOIs	https://doi.org/10.1016/j.bbrc.2020.03.138
State	Accepted/In press - Jan 1 2020

Keywords

Computational visual modeling
Convergent F-actin protrusions and cable network
Grhl3-KO mice
Multicellular rosette formation
Non-neural surface ectodermal cells
Posterior neuropore (PNP)

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure. / Zhou, Chengji J.; Ji, Yu; Reynolds, Kurt; McMahon, Moira; Garland, Michael A.; Zhang, Shuwen; Sun, Bo; Gu, Ran; Islam, Mohammad; Liu, Yue; Zhao, Tianyu; Hsu, Grace; Iwasa, Janet.

In: Biochemical and Biophysical Research Communications, 01.01.2020.

Research output: Contribution to journal › Article › peer-review

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abstract = "The mechanisms underlying mammalian neural tube closure remain poorly understood. We report a unique cellular process involving multicellular rosette formation, convergent cellular protrusions, and F-actin cable network of the non-neural surface ectodermal cells encircling the closure site of the posterior neuropore, which are demonstrated by scanning electron microscopy and genetic fate mapping analyses during mouse spinal neurulation. These unique cellular structures are severely disrupted in the surface ectodermal transcription factor Grhl3 mutants that exhibit fully penetrant spina bifida. We propose a novel model of mammalian neural tube closure driven by surface ectodermal dynamics, which is computationally visualized.",

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AU - Zhou, Chengji J.

AU - Ji, Yu

AU - Reynolds, Kurt

AU - McMahon, Moira

AU - Garland, Michael A.

AU - Zhang, Shuwen

AU - Sun, Bo

AU - Gu, Ran

AU - Islam, Mohammad

AU - Liu, Yue

AU - Zhao, Tianyu

AU - Hsu, Grace

AU - Iwasa, Janet

PY - 2020/1/1

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N2 - The mechanisms underlying mammalian neural tube closure remain poorly understood. We report a unique cellular process involving multicellular rosette formation, convergent cellular protrusions, and F-actin cable network of the non-neural surface ectodermal cells encircling the closure site of the posterior neuropore, which are demonstrated by scanning electron microscopy and genetic fate mapping analyses during mouse spinal neurulation. These unique cellular structures are severely disrupted in the surface ectodermal transcription factor Grhl3 mutants that exhibit fully penetrant spina bifida. We propose a novel model of mammalian neural tube closure driven by surface ectodermal dynamics, which is computationally visualized.

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KW - Computational visual modeling

KW - Convergent F-actin protrusions and cable network

KW - Grhl3-KO mice

KW - Multicellular rosette formation

KW - Non-neural surface ectodermal cells

KW - Posterior neuropore (PNP)

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Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure

Abstract

Keywords

ASJC Scopus subject areas

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