Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure

Chengji J. Zhou, Yu Ji, Kurt Reynolds, Moira McMahon, Michael A. Garland, Shuwen Zhang, Bo Sun, Ran Gu, Mohammad Islam, Yue Liu, Tianyu Zhao, Grace Hsu, Janet Iwasa

Research output: Contribution to journalArticle

Abstract

The mechanisms underlying mammalian neural tube closure remain poorly understood. We report a unique cellular process involving multicellular rosette formation, convergent cellular protrusions, and F-actin cable network of the non-neural surface ectodermal cells encircling the closure site of the posterior neuropore, which are demonstrated by scanning electron microscopy and genetic fate mapping analyses during mouse spinal neurulation. These unique cellular structures are severely disrupted in the surface ectodermal transcription factor Grhl3 mutants that exhibit fully penetrant spina bifida. We propose a novel model of mammalian neural tube closure driven by surface ectodermal dynamics, which is computationally visualized.

Original languageEnglish (US)
JournalBiochemical and Biophysical Research Communications
DOIs
StateAccepted/In press - Jan 1 2020

Keywords

  • Computational visual modeling
  • Convergent F-actin protrusions and cable network
  • Grhl3-KO mice
  • Multicellular rosette formation
  • Non-neural surface ectodermal cells
  • Posterior neuropore (PNP)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Zhou, C. J., Ji, Y., Reynolds, K., McMahon, M., Garland, M. A., Zhang, S., Sun, B., Gu, R., Islam, M., Liu, Y., Zhao, T., Hsu, G., & Iwasa, J. (Accepted/In press). Non-neural surface ectodermal rosette formation and F-actin dynamics drive mammalian neural tube closure. Biochemical and Biophysical Research Communications. https://doi.org/10.1016/j.bbrc.2020.03.138