Non-linear optical flow cytometry using a scanned, bessel beam light-sheet

Bradley B. Collier, Samir Awasthi, Deborah Lieu, James W Chan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Modern flow cytometry instruments have become vital tools for high-throughput analysis of single cells. However, as issues with the cellular labeling techniques often used in flow cytometry have become more of a concern, the development of label-free modalities for cellular analysis is increasingly desired. Non-linear optical phenomena (NLO) are of growing interest for label-free analysis because of the ability to measure the intrinsic optical response of biomolecules found in cells. We demonstrate that a light-sheet consisting of a scanned Bessel beam is an optimal excitation geometry for efficiently generating NLO signals in a microfluidic environment. The balance of photon density and cross-sectional area provided by the light-sheet allowed significantly larger two-photon fluorescence intensities to be measured in a model polystyrene microparticle system compared to measurements made using other excitation focal geometries, including a relaxed Gaussian excitation beam often used in conventional flow cytometers.

Original languageEnglish (US)
Article number10751
JournalScientific Reports
Volume5
DOIs
StatePublished - May 29 2015

ASJC Scopus subject areas

  • General

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