Abstract
Non-invasive in vivo functional optical imaging is demonstrated using high-speed, ultrahigh resolution optical coherence tomography (UHR-OCT). A high-speed, UHR-OCT system using spectral/Fourier domain detection was developed for functional imaging experiments in the rodent retina. Using a spectrally multiplexed superluminescent diode light source, imaging was performed with 2.8 μm resolution at a rate of 24,000 axial scans per second. OCT measurement protocols were designed to minimize noise sources that cause undesired fluctuations in the measured OCT signal. A white light stimulus was applied to the retina and the average reflectivity from each intraretinal layer was monitored over time using OCT. A white light stimulus induces a response consisting of an increase in the reflectance of the photoreceptor outer segments. To our knowledge, this is the first in vivo demonstration of functional imaging using OCT in the retina. Further systematic investigation will be required to fully characterize the observed optical changes. Eventually, this may prove to be an objective method for measuring photoreceptor function in the human retina.
| Original language | English (US) |
|---|---|
| Title of host publication | Ophthalmic Technologies XVI |
| Volume | 6138 |
| DOIs | |
| State | Published - Jun 30 2006 |
| Externally published | Yes |
| Event | Ophthalmic Technologies XVI - San Jose, CA, United States Duration: Jan 21 2006 → Jan 24 2006 |
Other
| Other | Ophthalmic Technologies XVI |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/21/06 → 1/24/06 |
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Keywords
- Fourier domain
- Functional
- Ophthalmology
- Optical Coherence Tomography
- Optophysiology
- Photoreceptors
- Rat
- Spectral
- Ultrahigh resolution
ASJC Scopus subject areas
- Engineering(all)
Cite this
Non-invasive in vivo measurement of retinal physiology with high-speed ultrahigh resolution OCT. / Srinivasan, Vivek; Wojtkowski, M.; Ko, T. H.; Duker, J. S.; Clermont, A.; Bursell, S.; Fujimoto, J. G.
Ophthalmic Technologies XVI. Vol. 6138 2006. 61380H.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Non-invasive in vivo measurement of retinal physiology with high-speed ultrahigh resolution OCT
AU - Srinivasan, Vivek
AU - Wojtkowski, M.
AU - Ko, T. H.
AU - Duker, J. S.
AU - Clermont, A.
AU - Bursell, S.
AU - Fujimoto, J. G.
PY - 2006/6/30
Y1 - 2006/6/30
N2 - Non-invasive in vivo functional optical imaging is demonstrated using high-speed, ultrahigh resolution optical coherence tomography (UHR-OCT). A high-speed, UHR-OCT system using spectral/Fourier domain detection was developed for functional imaging experiments in the rodent retina. Using a spectrally multiplexed superluminescent diode light source, imaging was performed with 2.8 μm resolution at a rate of 24,000 axial scans per second. OCT measurement protocols were designed to minimize noise sources that cause undesired fluctuations in the measured OCT signal. A white light stimulus was applied to the retina and the average reflectivity from each intraretinal layer was monitored over time using OCT. A white light stimulus induces a response consisting of an increase in the reflectance of the photoreceptor outer segments. To our knowledge, this is the first in vivo demonstration of functional imaging using OCT in the retina. Further systematic investigation will be required to fully characterize the observed optical changes. Eventually, this may prove to be an objective method for measuring photoreceptor function in the human retina.
AB - Non-invasive in vivo functional optical imaging is demonstrated using high-speed, ultrahigh resolution optical coherence tomography (UHR-OCT). A high-speed, UHR-OCT system using spectral/Fourier domain detection was developed for functional imaging experiments in the rodent retina. Using a spectrally multiplexed superluminescent diode light source, imaging was performed with 2.8 μm resolution at a rate of 24,000 axial scans per second. OCT measurement protocols were designed to minimize noise sources that cause undesired fluctuations in the measured OCT signal. A white light stimulus was applied to the retina and the average reflectivity from each intraretinal layer was monitored over time using OCT. A white light stimulus induces a response consisting of an increase in the reflectance of the photoreceptor outer segments. To our knowledge, this is the first in vivo demonstration of functional imaging using OCT in the retina. Further systematic investigation will be required to fully characterize the observed optical changes. Eventually, this may prove to be an objective method for measuring photoreceptor function in the human retina.
KW - Fourier domain
KW - Functional
KW - Ophthalmology
KW - Optical Coherence Tomography
KW - Optophysiology
KW - Photoreceptors
KW - Rat
KW - Spectral
KW - Ultrahigh resolution
UR - http://www.scopus.com/inward/record.url?scp=33745397725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745397725&partnerID=8YFLogxK
U2 - 10.1117/12.648923
DO - 10.1117/12.648923
M3 - Conference contribution
AN - SCOPUS:33745397725
SN - 0819461814
SN - 9780819461810
VL - 6138
BT - Ophthalmic Technologies XVI
ER -