Abstract
Light is a most versatile tool for investigating biological systems and phenomena; the range, non-destructiveness, spatial discrimination and speed of optical imaging are all important for investigating structure and function at the cellular, tissue or even whole organism level. In live biological imaging, where the technological requirements are heightened, other features of light, such as coherence and wavelength, are used to generate the additional contrast and resolution needed. We report here recent improvements in our ability to image biological specimens optically, focusing on (a) spectral resolution and the related image processing issues, and (b) tomographic three-dimensional fluorescence imaging in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 89-102 |
| Number of pages | 14 |
| Journal | Computerized Medical Imaging and Graphics |
| Volume | 22 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 1998 |
| Externally published | Yes |
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Keywords
- Acousto-optic tunable filters
- Back-projection
- Fluorescence
- Fourier spectroscopy
- Mesoscopy
- Microscopy
- Processing
- Reconstruction
- Spectral imaging
- Tomography
ASJC Scopus subject areas
- Computer Science Applications
- Radiology Nuclear Medicine and imaging
Cite this
Non-invasive image acquisition and advanced processing in optical bioimaging. / Farkas, Daniel L.; Du, Congwu; Fisher, Gregory W.; Lau, Christopher; Niu, Wenhua; Wachman, Elliot S.; Levenson, Richard M.
In: Computerized Medical Imaging and Graphics, Vol. 22, No. 2, 03.1998, p. 89-102.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Non-invasive image acquisition and advanced processing in optical bioimaging
AU - Farkas, Daniel L.
AU - Du, Congwu
AU - Fisher, Gregory W.
AU - Lau, Christopher
AU - Niu, Wenhua
AU - Wachman, Elliot S.
AU - Levenson, Richard M
PY - 1998/3
Y1 - 1998/3
N2 - Light is a most versatile tool for investigating biological systems and phenomena; the range, non-destructiveness, spatial discrimination and speed of optical imaging are all important for investigating structure and function at the cellular, tissue or even whole organism level. In live biological imaging, where the technological requirements are heightened, other features of light, such as coherence and wavelength, are used to generate the additional contrast and resolution needed. We report here recent improvements in our ability to image biological specimens optically, focusing on (a) spectral resolution and the related image processing issues, and (b) tomographic three-dimensional fluorescence imaging in vivo.
AB - Light is a most versatile tool for investigating biological systems and phenomena; the range, non-destructiveness, spatial discrimination and speed of optical imaging are all important for investigating structure and function at the cellular, tissue or even whole organism level. In live biological imaging, where the technological requirements are heightened, other features of light, such as coherence and wavelength, are used to generate the additional contrast and resolution needed. We report here recent improvements in our ability to image biological specimens optically, focusing on (a) spectral resolution and the related image processing issues, and (b) tomographic three-dimensional fluorescence imaging in vivo.
KW - Acousto-optic tunable filters
KW - Back-projection
KW - Fluorescence
KW - Fourier spectroscopy
KW - Mesoscopy
KW - Microscopy
KW - Processing
KW - Reconstruction
KW - Spectral imaging
KW - Tomography
UR - http://www.scopus.com/inward/record.url?scp=0032029043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032029043&partnerID=8YFLogxK
U2 - 10.1016/S0895-6111(98)00011-1
DO - 10.1016/S0895-6111(98)00011-1
M3 - Article
C2 - 9719850
AN - SCOPUS:0032029043
VL - 22
SP - 89
EP - 102
JO - Computerized Medical Imaging and Graphics
JF - Computerized Medical Imaging and Graphics
SN - 0895-6111
IS - 2
ER -