Nod-like receptors are critical for gut–brain axis signalling in mice

Matteo M. Pusceddu, Mariana Barboza, Ciara E. Keogh, Melinda Schneider, Patricia Stokes, Jessica A. Sladek, Hyun Jung D. Kim, Cristina Torres-Fuentes, Lily R. Goldfild, Shane E. Gillis, Ingrid Brust-Mascher, Gonzalo Rabasa, Kyle A. Wong, Carlito Lebrilla, Mariana X. Byndloss, Charles Maisonneuve, Andreas J. Bäumler, Dana J. Philpott, Richard L. Ferrero, Kim E. BarrettColin Reardon, Mélanie G. Gareau

Research output: Contribution to journalArticle

Abstract

Key points: •Nucleotide binding oligomerization domain (Nod)-like receptors regulate cognition, anxiety and hypothalamic–pituitary–adrenal axis activation. •Nod-like receptors regulate central and peripheral serotonergic biology. •Nod-like receptors are important for maintenance of gastrointestinal physiology. •Intestinal epithelial cell expression of Nod1 receptors regulate behaviour. Abstract: Gut–brain axis signalling is critical for maintaining health and homeostasis. Stressful life events can impact gut–brain signalling, leading to altered mood, cognition and intestinal dysfunction. In the present study, we identified nucleotide binding oligomerization domain (Nod)-like receptors (NLR), Nod1 and Nod2, as novel regulators for gut–brain signalling. NLR are innate immune pattern recognition receptors expressed in the gut and brain, and are important in the regulation of gastrointestinal physiology. We found that mice deficient in both Nod1 and Nod2 (NodDKO) demonstrate signs of stress-induced anxiety, cognitive impairment and depression in the context of a hyperactive hypothalamic–pituitary–adrenal axis. These deficits were coupled with impairments in the serotonergic pathway in the brain, decreased hippocampal cell proliferation and immature neurons, as well as reduced neural activation. In addition, NodDKO mice had increased gastrointestinal permeability and altered serotonin signalling in the gut following exposure to acute stress. Administration of the selective serotonin reuptake inhibitor, fluoxetine, abrogated behavioural impairments and restored serotonin signalling. We also identified that intestinal epithelial cell-specific deletion of Nod1 (VilCre+Nod1f/f), but not Nod2, increased susceptibility to stress-induced anxiety-like behaviour and cognitive impairment following exposure to stress. Together, these data suggest that intestinal epithelial NLR are novel modulators of gut–brain communication and may serve as potential novel therapeutic targets for the treatment of gut–brain disorders.

Original languageEnglish (US)
Pages (from-to)5777-5797
Number of pages21
JournalJournal of Physiology
Volume597
Issue number24
DOIs
StatePublished - Dec 1 2019

Fingerprint

Nucleotides
Anxiety
Cognition
Serotonin
Epithelial Cells
Pattern Recognition Receptors
Fluoxetine
Serotonin Uptake Inhibitors
Brain
Permeability
Homeostasis
Communication
Maintenance
Cell Proliferation
Depression
Neurons
Health
Cognitive Dysfunction
Therapeutics

Keywords

  • 5-HT system
  • anxiety
  • cognition
  • depression
  • HPA axis
  • intestinal physiology
  • microbiota-gut–brain axis
  • neurogenesis
  • NLR
  • stress

ASJC Scopus subject areas

  • Physiology

Cite this

Pusceddu, M. M., Barboza, M., Keogh, C. E., Schneider, M., Stokes, P., Sladek, J. A., ... Gareau, M. G. (2019). Nod-like receptors are critical for gut–brain axis signalling in mice. Journal of Physiology, 597(24), 5777-5797. https://doi.org/10.1113/JP278640

Nod-like receptors are critical for gut–brain axis signalling in mice. / Pusceddu, Matteo M.; Barboza, Mariana; Keogh, Ciara E.; Schneider, Melinda; Stokes, Patricia; Sladek, Jessica A.; Kim, Hyun Jung D.; Torres-Fuentes, Cristina; Goldfild, Lily R.; Gillis, Shane E.; Brust-Mascher, Ingrid; Rabasa, Gonzalo; Wong, Kyle A.; Lebrilla, Carlito; Byndloss, Mariana X.; Maisonneuve, Charles; Bäumler, Andreas J.; Philpott, Dana J.; Ferrero, Richard L.; Barrett, Kim E.; Reardon, Colin; Gareau, Mélanie G.

In: Journal of Physiology, Vol. 597, No. 24, 01.12.2019, p. 5777-5797.

Research output: Contribution to journalArticle

Pusceddu, MM, Barboza, M, Keogh, CE, Schneider, M, Stokes, P, Sladek, JA, Kim, HJD, Torres-Fuentes, C, Goldfild, LR, Gillis, SE, Brust-Mascher, I, Rabasa, G, Wong, KA, Lebrilla, C, Byndloss, MX, Maisonneuve, C, Bäumler, AJ, Philpott, DJ, Ferrero, RL, Barrett, KE, Reardon, C & Gareau, MG 2019, 'Nod-like receptors are critical for gut–brain axis signalling in mice', Journal of Physiology, vol. 597, no. 24, pp. 5777-5797. https://doi.org/10.1113/JP278640
Pusceddu MM, Barboza M, Keogh CE, Schneider M, Stokes P, Sladek JA et al. Nod-like receptors are critical for gut–brain axis signalling in mice. Journal of Physiology. 2019 Dec 1;597(24):5777-5797. https://doi.org/10.1113/JP278640
Pusceddu, Matteo M. ; Barboza, Mariana ; Keogh, Ciara E. ; Schneider, Melinda ; Stokes, Patricia ; Sladek, Jessica A. ; Kim, Hyun Jung D. ; Torres-Fuentes, Cristina ; Goldfild, Lily R. ; Gillis, Shane E. ; Brust-Mascher, Ingrid ; Rabasa, Gonzalo ; Wong, Kyle A. ; Lebrilla, Carlito ; Byndloss, Mariana X. ; Maisonneuve, Charles ; Bäumler, Andreas J. ; Philpott, Dana J. ; Ferrero, Richard L. ; Barrett, Kim E. ; Reardon, Colin ; Gareau, Mélanie G. / Nod-like receptors are critical for gut–brain axis signalling in mice. In: Journal of Physiology. 2019 ; Vol. 597, No. 24. pp. 5777-5797.
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AU - Stokes, Patricia

AU - Sladek, Jessica A.

AU - Kim, Hyun Jung D.

AU - Torres-Fuentes, Cristina

AU - Goldfild, Lily R.

AU - Gillis, Shane E.

AU - Brust-Mascher, Ingrid

AU - Rabasa, Gonzalo

AU - Wong, Kyle A.

AU - Lebrilla, Carlito

AU - Byndloss, Mariana X.

AU - Maisonneuve, Charles

AU - Bäumler, Andreas J.

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