Nocardia crassostreae sp. nov., the causal agent of nocardiosis in Pacific oysters

Carolyn S. Friedman, Blaine L. Beaman, Jongsik Chun, Michael Goodfellow, Arthur Gee, Ronald Hedrick

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Seven strains of bacteria were isolated from Pacific oysters, Crassostrea gigas, with a focal or systemic disease. The strains were aerobic, Gram-positive, acid-fast, produced a mycelium which fragmented into irregular rod-like elements, had a peptidoglycan containing meso- diaminopimelic acid, arabinose and galactose as major sugars, mycolic acids with 46-58 carbon atoms and G+C-rich DNA. All of these properties are consistent with the classification of the organisms in the genus Nocardia. A partial sequence of the 16S rRNA gene of isolate NB4H was determined following isolation and cloning of the PCR-amplified gene. The sequence was aligned with those of representative mycolic-acid-containing taxa and a phylogenetic tree was generated using the neighbour-joining method. It was evident from the phylogenetic tree that the three strains tested, RB1, OB3P and NB4H, were identical and belonged to the Nocardia otitidiscaviarum rRNA sub-group. The biochemical, chemical, morphological and physiological properties of the isolates were also essentially identical and served to distinguish them from representative nocardiae. It is, therefore, proposed that the strains isolated from the diseased Pacific oysters be assigned to a new species, Nocardia crassostreae. The type strain is NB4H (= ATCC 700418).

Original languageEnglish (US)
Pages (from-to)237-246
Number of pages10
JournalInternational Journal of Systematic Bacteriology
Volume48
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

Crassostrea
Nocardia Infections
Nocardia
Ostreidae
Crassostrea gigas
Mycolic Acids
new species
acid
acids
Diaminopimelic Acid
Nocardia otitidiscaviarum
phylogenetics
Sugar Acids
Arabinose
Peptidoglycan
ribosomal RNA
gene
Mycelium
Galactose
rRNA Genes

Keywords

  • Nocardia crassostreae sp. nov.
  • Nocardiosis
  • Pacific oysters

ASJC Scopus subject areas

  • Microbiology
  • Immunology

Cite this

Nocardia crassostreae sp. nov., the causal agent of nocardiosis in Pacific oysters. / Friedman, Carolyn S.; Beaman, Blaine L.; Chun, Jongsik; Goodfellow, Michael; Gee, Arthur; Hedrick, Ronald.

In: International Journal of Systematic Bacteriology, Vol. 48, No. 1, 01.01.1998, p. 237-246.

Research output: Contribution to journalArticle

Friedman, Carolyn S. ; Beaman, Blaine L. ; Chun, Jongsik ; Goodfellow, Michael ; Gee, Arthur ; Hedrick, Ronald. / Nocardia crassostreae sp. nov., the causal agent of nocardiosis in Pacific oysters. In: International Journal of Systematic Bacteriology. 1998 ; Vol. 48, No. 1. pp. 237-246.
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