Abstract
Seven strains of bacteria were isolated from Pacific oysters, Crassostrea gigas, with a focal or systemic disease. The strains were aerobic, Gram-positive, acid-fast, produced a mycelium which fragmented into irregular rod-like elements, had a peptidoglycan containing meso- diaminopimelic acid, arabinose and galactose as major sugars, mycolic acids with 46-58 carbon atoms and G+C-rich DNA. All of these properties are consistent with the classification of the organisms in the genus Nocardia. A partial sequence of the 16S rRNA gene of isolate NB4H was determined following isolation and cloning of the PCR-amplified gene. The sequence was aligned with those of representative mycolic-acid-containing taxa and a phylogenetic tree was generated using the neighbour-joining method. It was evident from the phylogenetic tree that the three strains tested, RB1, OB3P and NB4H, were identical and belonged to the Nocardia otitidiscaviarum rRNA sub-group. The biochemical, chemical, morphological and physiological properties of the isolates were also essentially identical and served to distinguish them from representative nocardiae. It is, therefore, proposed that the strains isolated from the diseased Pacific oysters be assigned to a new species, Nocardia crassostreae. The type strain is NB4H (= ATCC 700418).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 237-246 |
| Number of pages | 10 |
| Journal | International Journal of Systematic Bacteriology |
| Volume | 48 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1998 |
Fingerprint
Keywords
- Nocardia crassostreae sp. nov.
- Nocardiosis
- Pacific oysters
ASJC Scopus subject areas
- Microbiology
- Immunology
Cite this
Nocardia crassostreae sp. nov., the causal agent of nocardiosis in Pacific oysters. / Friedman, Carolyn S.; Beaman, Blaine L.; Chun, Jongsik; Goodfellow, Michael; Gee, Arthur; Hedrick, Ronald.
In: International Journal of Systematic Bacteriology, Vol. 48, No. 1, 01.01.1998, p. 237-246.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nocardia crassostreae sp. nov., the causal agent of nocardiosis in Pacific oysters
AU - Friedman, Carolyn S.
AU - Beaman, Blaine L.
AU - Chun, Jongsik
AU - Goodfellow, Michael
AU - Gee, Arthur
AU - Hedrick, Ronald
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Seven strains of bacteria were isolated from Pacific oysters, Crassostrea gigas, with a focal or systemic disease. The strains were aerobic, Gram-positive, acid-fast, produced a mycelium which fragmented into irregular rod-like elements, had a peptidoglycan containing meso- diaminopimelic acid, arabinose and galactose as major sugars, mycolic acids with 46-58 carbon atoms and G+C-rich DNA. All of these properties are consistent with the classification of the organisms in the genus Nocardia. A partial sequence of the 16S rRNA gene of isolate NB4H was determined following isolation and cloning of the PCR-amplified gene. The sequence was aligned with those of representative mycolic-acid-containing taxa and a phylogenetic tree was generated using the neighbour-joining method. It was evident from the phylogenetic tree that the three strains tested, RB1, OB3P and NB4H, were identical and belonged to the Nocardia otitidiscaviarum rRNA sub-group. The biochemical, chemical, morphological and physiological properties of the isolates were also essentially identical and served to distinguish them from representative nocardiae. It is, therefore, proposed that the strains isolated from the diseased Pacific oysters be assigned to a new species, Nocardia crassostreae. The type strain is NB4H (= ATCC 700418).
AB - Seven strains of bacteria were isolated from Pacific oysters, Crassostrea gigas, with a focal or systemic disease. The strains were aerobic, Gram-positive, acid-fast, produced a mycelium which fragmented into irregular rod-like elements, had a peptidoglycan containing meso- diaminopimelic acid, arabinose and galactose as major sugars, mycolic acids with 46-58 carbon atoms and G+C-rich DNA. All of these properties are consistent with the classification of the organisms in the genus Nocardia. A partial sequence of the 16S rRNA gene of isolate NB4H was determined following isolation and cloning of the PCR-amplified gene. The sequence was aligned with those of representative mycolic-acid-containing taxa and a phylogenetic tree was generated using the neighbour-joining method. It was evident from the phylogenetic tree that the three strains tested, RB1, OB3P and NB4H, were identical and belonged to the Nocardia otitidiscaviarum rRNA sub-group. The biochemical, chemical, morphological and physiological properties of the isolates were also essentially identical and served to distinguish them from representative nocardiae. It is, therefore, proposed that the strains isolated from the diseased Pacific oysters be assigned to a new species, Nocardia crassostreae. The type strain is NB4H (= ATCC 700418).
KW - Nocardia crassostreae sp. nov.
KW - Nocardiosis
KW - Pacific oysters
UR - http://www.scopus.com/inward/record.url?scp=0031952879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031952879&partnerID=8YFLogxK
U2 - 10.1099/00207713-48-1-237
DO - 10.1099/00207713-48-1-237
M3 - Article
C2 - 9542093
AN - SCOPUS:0031952879
VL - 48
SP - 237
EP - 246
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 1
ER -