No evidence of Helicobacter pylori sequences in pancreatic juices of patients affected by chronic pancreatitis

C. Di Campli, R. Nocente, G. Costamagna, N. Gentiloni, R. Burioni, J. Wu, A. Armuzzi, Mark A Zern, G. Gasbarrini, A. Gasbarrini

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Background: The course of chronic pancreatitis is often unpredictable and many factors are likely to be involved in the progression of the disease. In physiological condition, pancreatic juice exerts significant antibacterial activity, which is impaired in patients with chronic pancreatitis. Aim: Hypothesizing that Helicobacter pylori could, in these conditions, lead to an ascending infection, we aimed to assess the presence of H. pylori sequences in pancreatic juices of patients with chronic pancreatitis. Methods: 40 patients (mean age 52 ± 3 yr) with alcoholic chronic pancreatitis and H. pylori infection were examined. Pancreatic juices were collected during endoscopic retrograde cholangiopancreatography. Using polymerase chain reaction (PCR) with two primers homologous to a portion of urease-C gene, H. pylori DNA was detected. Gastric biopsies, microscopically positive to H. pylori were used as positive controls. Results: All gastric biopsies produced H. pylori-specific DNA products. Conversely, no H. pylori urease-C gene sequences have been detected in any of the pancreatic juices. Conclusion: Our data suggest that the impaired antibacterial activity of pancreatic juices in patients affected by chronic pancreatitis does not have a permissive role for a superimposing H. pylori infection in the pancreas. The possibility that Helicobacter species other than pylori may be involved in a superimposing infection requires further investigation.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalInternational Journal of Pancreatology
Volume28
Issue number3
StatePublished - 2000
Externally publishedYes

Keywords

  • Chronic pancreatitis
  • Helicobacter pylori
  • Pancreatic juice
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology
  • Oncology

Fingerprint Dive into the research topics of 'No evidence of Helicobacter pylori sequences in pancreatic juices of patients affected by chronic pancreatitis'. Together they form a unique fingerprint.

  • Cite this

    Di Campli, C., Nocente, R., Costamagna, G., Gentiloni, N., Burioni, R., Wu, J., Armuzzi, A., Zern, M. A., Gasbarrini, G., & Gasbarrini, A. (2000). No evidence of Helicobacter pylori sequences in pancreatic juices of patients affected by chronic pancreatitis. International Journal of Pancreatology, 28(3), 181-185.