NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content

Andrew M. Chiu, Jiejie Wang, Michael P. Fiske, P. Hubalkova, Levi Barse, John Gray, Antonio Sanz-Clemente

Research output: Contribution to journalArticle

Abstract

In mature neurons, postsynaptic N-methyl-D-aspartate receptors (NMDARs) are segregated into two populations, synaptic and extrasynaptic, which differ in localization, function, and associated intracellular cascades. These two pools are connected via lateral diffusion, and receptor exchange between them modulates synaptic NMDAR content. Here, we identify the phosphorylation of the PDZ-ligand of the GluN2B subunit of NMDARs (at S1480) as a critical determinant in dynamically controlling NMDAR synaptic content. We find that phosphorylation of GluN2B at S1480 maintains NMDARs at extrasynaptic membranes as part of a protein complex containing protein phosphatase 1 (PP1). Global activation of NMDARs leads to the activation of PP1, which mediates dephosphorylation of GluN2B at S1480 to promote an increase in synaptic NMDAR content. Thus, PP1-mediated dephosphorylation of the GluN2B PDZ-ligand modulates the synaptic expression of NMDARs in mature neurons in an activity-dependent manner, a process with profound consequences for synaptic and structural plasticity, metaplasticity, and synaptic neurotransmission. The dynamic regulation of synaptically expressed NMDA receptors (NMDARs) is essential for synaptic function. Here, Chiu et al. describe a mechanism controlling this process in mature neurons by showing that increases in NMDAR synaptic content are driven by PP1-mediated dephosphorylation of extrasynaptic NMDARs within their GluN2B PDZ-ligands.

Original languageEnglish (US)
Pages (from-to)332-341.e5
JournalCell Reports
Volume28
Issue number2
DOIs
StatePublished - Jul 9 2019

Fingerprint

Protein Phosphatase 1
N-Methyl-D-Aspartate Receptors
Neurons
Phosphorylation
Ligands
Chemical activation
Neuronal Plasticity
Synaptic Transmission
Plasticity

Keywords

  • dephosphorylation
  • extrasynaptic NMDAR
  • GluN2B
  • NMDA receptors
  • NMDAR synaptic content
  • PP1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chiu, A. M., Wang, J., Fiske, M. P., Hubalkova, P., Barse, L., Gray, J., & Sanz-Clemente, A. (2019). NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. Cell Reports, 28(2), 332-341.e5. https://doi.org/10.1016/j.celrep.2019.06.030

NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. / Chiu, Andrew M.; Wang, Jiejie; Fiske, Michael P.; Hubalkova, P.; Barse, Levi; Gray, John; Sanz-Clemente, Antonio.

In: Cell Reports, Vol. 28, No. 2, 09.07.2019, p. 332-341.e5.

Research output: Contribution to journalArticle

Chiu, AM, Wang, J, Fiske, MP, Hubalkova, P, Barse, L, Gray, J & Sanz-Clemente, A 2019, 'NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content', Cell Reports, vol. 28, no. 2, pp. 332-341.e5. https://doi.org/10.1016/j.celrep.2019.06.030
Chiu, Andrew M. ; Wang, Jiejie ; Fiske, Michael P. ; Hubalkova, P. ; Barse, Levi ; Gray, John ; Sanz-Clemente, Antonio. / NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. In: Cell Reports. 2019 ; Vol. 28, No. 2. pp. 332-341.e5.
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