Abstract
The c-fos and junB immediate early genes (IEGs) were induced in neurons of the medial and ventral striatum following administration of morphine. The striatal induction of c-fos and junB mRNA and Fos protein was blocked by naloxone, the D1 dopamine (DA) receptor antagonists, SCH23390 and SCH39166, and the N-methyl-D-aspartate (NMDA) glutamate receptor antagonist, MK801. SCH23390 and MK801 did not block morphine induction of c-fos and junB in septum. Since the pattern of the morphine induction of c-fos and junB in striatum and nucleus accumbens was similar to that observed with cocaine and amphetamine [2,18,45,51], these data support current concepts that limbic striatum and nucleus accumbens are among the brain regions that mediate drug abuse [9,10,23,27,49]. If it is true that D1 receptors activate the CRE (cyclase response element) and NMDA receptors activate the SRE (serum response element) in the c-fos promoter [1], then this data suggests that serial activation of μ opiate, NMDA and D1 receptors on different neurons is required to induced Fos in striatal neurons with D1 receptors. Moreover, concurrent activation of NMDA and D1 receptors is required for Fos induction in striatal neurons. The Fos induced by this simultaneous activation of NMDA and D1 receptors should lead to long-term changes of gene expression that might also be involved in changes of brain circuits that could form the basis for 'memories' relating to prior exposure to addictive drugs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 225-230 |
| Number of pages | 6 |
| Journal | Behavioural Brain Research |
| Volume | 66 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Jan 23 1995 |
| Externally published | Yes |
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Keywords
- Addiction
- c-fos
- Dopamine receptor
- Glutamate
- Immediate early gene
- junB
- Memory
- Morphine
- NMDA receptor
- Striatum
- Transcription factor
ASJC Scopus subject areas
- Behavioral Neuroscience
Cite this
NMDA and D1 receptors mediate induction of c-fos and junB genes in striatum following morphine administration : implications for studies of memory. / Sharp, Frank R; Liu, Jialing; Nickolenko, Jeremy; Bontempi, Bruno.
In: Behavioural Brain Research, Vol. 66, No. 1-2, 23.01.1995, p. 225-230.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - NMDA and D1 receptors mediate induction of c-fos and junB genes in striatum following morphine administration
T2 - implications for studies of memory
AU - Sharp, Frank R
AU - Liu, Jialing
AU - Nickolenko, Jeremy
AU - Bontempi, Bruno
PY - 1995/1/23
Y1 - 1995/1/23
N2 - The c-fos and junB immediate early genes (IEGs) were induced in neurons of the medial and ventral striatum following administration of morphine. The striatal induction of c-fos and junB mRNA and Fos protein was blocked by naloxone, the D1 dopamine (DA) receptor antagonists, SCH23390 and SCH39166, and the N-methyl-D-aspartate (NMDA) glutamate receptor antagonist, MK801. SCH23390 and MK801 did not block morphine induction of c-fos and junB in septum. Since the pattern of the morphine induction of c-fos and junB in striatum and nucleus accumbens was similar to that observed with cocaine and amphetamine [2,18,45,51], these data support current concepts that limbic striatum and nucleus accumbens are among the brain regions that mediate drug abuse [9,10,23,27,49]. If it is true that D1 receptors activate the CRE (cyclase response element) and NMDA receptors activate the SRE (serum response element) in the c-fos promoter [1], then this data suggests that serial activation of μ opiate, NMDA and D1 receptors on different neurons is required to induced Fos in striatal neurons with D1 receptors. Moreover, concurrent activation of NMDA and D1 receptors is required for Fos induction in striatal neurons. The Fos induced by this simultaneous activation of NMDA and D1 receptors should lead to long-term changes of gene expression that might also be involved in changes of brain circuits that could form the basis for 'memories' relating to prior exposure to addictive drugs.
AB - The c-fos and junB immediate early genes (IEGs) were induced in neurons of the medial and ventral striatum following administration of morphine. The striatal induction of c-fos and junB mRNA and Fos protein was blocked by naloxone, the D1 dopamine (DA) receptor antagonists, SCH23390 and SCH39166, and the N-methyl-D-aspartate (NMDA) glutamate receptor antagonist, MK801. SCH23390 and MK801 did not block morphine induction of c-fos and junB in septum. Since the pattern of the morphine induction of c-fos and junB in striatum and nucleus accumbens was similar to that observed with cocaine and amphetamine [2,18,45,51], these data support current concepts that limbic striatum and nucleus accumbens are among the brain regions that mediate drug abuse [9,10,23,27,49]. If it is true that D1 receptors activate the CRE (cyclase response element) and NMDA receptors activate the SRE (serum response element) in the c-fos promoter [1], then this data suggests that serial activation of μ opiate, NMDA and D1 receptors on different neurons is required to induced Fos in striatal neurons with D1 receptors. Moreover, concurrent activation of NMDA and D1 receptors is required for Fos induction in striatal neurons. The Fos induced by this simultaneous activation of NMDA and D1 receptors should lead to long-term changes of gene expression that might also be involved in changes of brain circuits that could form the basis for 'memories' relating to prior exposure to addictive drugs.
KW - Addiction
KW - c-fos
KW - Dopamine receptor
KW - Glutamate
KW - Immediate early gene
KW - junB
KW - Memory
KW - Morphine
KW - NMDA receptor
KW - Striatum
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=0028979460&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028979460&partnerID=8YFLogxK
U2 - 10.1016/0166-4328(94)00146-7
DO - 10.1016/0166-4328(94)00146-7
M3 - Article
C2 - 7755894
AN - SCOPUS:0028979460
VL - 66
SP - 225
EP - 230
JO - Behavioural Brain Research
JF - Behavioural Brain Research
SN - 0166-4328
IS - 1-2
ER -