NLS peptide conjugated molecular beacons for visualizing nuclear RNA in living cells

Nitin Nitin, Gang Bao

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Imaging the expression and localization of RNAs in live-cell nucleus can provide important information on RNA synthesis, processing, and transport. Here, we report the development of a bifunctional molecular beacon (NLS-MB) composed of a single nuclear localization sequence (NLS) peptide conjugated to a molecular beacon for efficient delivery and imaging of endogenous RNAs in the nuclei of living cells. We characterized the NLS-MBs by comparing their signal-to-noise ratios with unmodified molecular beacons and determined their efficiency of nuclear import. We demonstrated the specificity and sensitivity of the method by observing in living cells the localization and colocalization of small nuclear RNAs (snRNA) U1 and U2 at discrete foci in the nucleoplasm, and the localization of small nucleolar RNA U3 in the nucleolus. These snRNAs were chosen because of their essential roles in RNA biogenesis. The results were validated using in situ hybridization as positive control and random beacons as negative control. This novel approach may be applied to imaging other nuclear RNAs and pre-mRNAs in living cells.

Original languageEnglish (US)
Pages (from-to)2205-2211
Number of pages7
JournalBioconjugate Chemistry
Volume19
Issue number11
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Nuclear RNA
RNA
Peptides
Cells
Cell Nucleus
Imaging techniques
Cell Nucleus Active Transport
RNA Precursors
Signal-To-Noise Ratio
In Situ Hybridization
Signal to noise ratio
Sensitivity and Specificity
Processing

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

NLS peptide conjugated molecular beacons for visualizing nuclear RNA in living cells. / Nitin, Nitin; Bao, Gang.

In: Bioconjugate Chemistry, Vol. 19, No. 11, 11.2008, p. 2205-2211.

Research output: Contribution to journalArticle

Nitin, Nitin ; Bao, Gang. / NLS peptide conjugated molecular beacons for visualizing nuclear RNA in living cells. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 11. pp. 2205-2211.
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