Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells

R. Castagna, P. A. Davis, V. T. Vasu, K. Soucek, Carroll E Cross, L. Greci, G. Valacchi

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.

Original languageEnglish (US)
Pages (from-to)365-370
Number of pages6
JournalToxicology in Vitro
Volume23
Issue number3
DOIs
StatePublished - Apr 2009

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Keywords

  • IL-8
  • Lung
  • Ozone
  • TEMPO

ASJC Scopus subject areas

  • Toxicology

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