Abstract
Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 365-370 |
| Number of pages | 6 |
| Journal | Toxicology in Vitro |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| State | Published - Apr 2009 |
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Keywords
- IL-8
- Lung
- Ozone
- TEMPO
ASJC Scopus subject areas
- Toxicology
Cite this
Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells. / Castagna, R.; Davis, P. A.; Vasu, V. T.; Soucek, K.; Cross, Carroll E; Greci, L.; Valacchi, G.
In: Toxicology in Vitro, Vol. 23, No. 3, 04.2009, p. 365-370.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells
AU - Castagna, R.
AU - Davis, P. A.
AU - Vasu, V. T.
AU - Soucek, K.
AU - Cross, Carroll E
AU - Greci, L.
AU - Valacchi, G.
PY - 2009/4
Y1 - 2009/4
N2 - Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.
AB - Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.
KW - IL-8
KW - Lung
KW - Ozone
KW - TEMPO
UR - http://www.scopus.com/inward/record.url?scp=60549106822&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60549106822&partnerID=8YFLogxK
U2 - 10.1016/j.tiv.2008.12.016
DO - 10.1016/j.tiv.2008.12.016
M3 - Article
C2 - 19162161
AN - SCOPUS:60549106822
VL - 23
SP - 365
EP - 370
JO - Toxicology in Vitro
JF - Toxicology in Vitro
SN - 0887-2333
IS - 3
ER -