Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells

R. Castagna; P. A. Davis; V. T. Vasu; K. Soucek; Carroll E Cross; L. Greci; G. Valacchi

doi:10.1016/j.tiv.2008.12.016

Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells

R. Castagna, P. A. Davis, V. T. Vasu, K. Soucek, Carroll E Cross, L. Greci, G. Valacchi

Pulmonary, Critical Care, and Sleep Medicine

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Exposure to high levels of ozone (O₃) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O₃-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O₃-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O₃ and/or O₃-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O₃ and/or its cytotoxic products and may provide protections against O₃-induced biotoxicities.

Original language	English (US)
Pages (from-to)	365-370
Number of pages	6
Journal	Toxicology in Vitro
Volume	23
Issue number	3
DOIs	https://doi.org/10.1016/j.tiv.2008.12.016
State	Published - Apr 2009

Keywords

IL-8
Lung
Ozone
TEMPO

ASJC Scopus subject areas

Toxicology

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title = "Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells",

abstract = "Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.",

keywords = "IL-8, Lung, Ozone, TEMPO",

author = "R. Castagna and Davis, {P. A.} and Vasu, {V. T.} and K. Soucek and Cross, {Carroll E} and L. Greci and G. Valacchi",

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AU - Castagna, R.

AU - Davis, P. A.

AU - Vasu, V. T.

AU - Soucek, K.

AU - Cross, Carroll E

AU - Greci, L.

AU - Valacchi, G.

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N2 - Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.

AB - Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.

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KW - Ozone

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