Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells

R. Castagna, P. A. Davis, V. T. Vasu, K. Soucek, Carroll E Cross, L. Greci, G. Valacchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Exposure to high levels of ozone (O3) damages respiratory tract epithelial cells. This research evaluated the ability of TEMPO (2,2,6,6-tetramethylpiperidine-N-oxyl), a stable nitroxide free radical, to decrease O3-mediated injury to a respiratory tract-derived cell line (A549 cells) by monitoring in this cell system the interleukine-8 (IL-8) production. TEMPO reduced O3-induced IL-8 production in A549 cells, as evidenced by PCR analysis, Western blot and ELISA assays. This behaviour is explainable on the basis of the reactivity between TEMPO with O3 and/or O3-derived free radicals in biological systems. The study provides evidence that TEMPO reacts with O3 and/or its cytotoxic products and may provide protections against O3-induced biotoxicities.

Original languageEnglish (US)
Pages (from-to)365-370
Number of pages6
JournalToxicology in Vitro
Volume23
Issue number3
DOIs
StatePublished - Apr 2009

Fingerprint

Ozone
Chemokines
Epithelial Cells
Lung
Respiratory System
Free Radicals
Biological systems
Assays
Western Blotting
Enzyme-Linked Immunosorbent Assay
Cells
Polymerase Chain Reaction
nitroxyl
tempol
Monitoring
Wounds and Injuries
Research
A549 Cells

Keywords

  • IL-8
  • Lung
  • Ozone
  • TEMPO

ASJC Scopus subject areas

  • Toxicology

Cite this

Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells. / Castagna, R.; Davis, P. A.; Vasu, V. T.; Soucek, K.; Cross, Carroll E; Greci, L.; Valacchi, G.

In: Toxicology in Vitro, Vol. 23, No. 3, 04.2009, p. 365-370.

Research output: Contribution to journalArticle

Castagna, R. ; Davis, P. A. ; Vasu, V. T. ; Soucek, K. ; Cross, Carroll E ; Greci, L. ; Valacchi, G. / Nitroxide radical TEMPO reduces ozone-induced chemokine IL-8 production in lung epithelial cells. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 3. pp. 365-370.
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