Nitrogen fixation in a landrace of maize is supported by a mucilage-associated diazotrophic microbiota

Allen Van Deynze, Pablo Zamora, Pierre Marc Delaux, Cristobal Heitmann, Dhileepkumar Jayaraman, Shanmugam Rajasekar, Danielle Graham, Junko Maeda, Donald Gibson, Kevin D. Schwartz, Alison M. Berry, Srijak Bhatnagar, Guillaume Jospin, Aaron Darling, Richard Jeannotte, Javier Lopez, Bart C Weimer, Jonathan A Eisen, Howard Yana Shapiro, Jean Michel AnéAlan B. Bennett

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Plants are associated with a complex microbiota that contributes to nutrient acquisition, plant growth, and plant defense. Nitrogen-fixing microbial associations are efficient and well characterized in legumes but are limited in cereals, including maize. We studied an indigenous landrace of maize grown in nitrogen-depleted soils in the Sierra Mixe region of Oaxaca, Mexico. This landrace is characterized by the extensive development of aerial roots that secrete a carbohydrate-rich mucilage. Analysis of the mucilage microbiota indicated that it was enriched in taxa for which many known species are diazotrophic, was enriched for homologs of genes encoding nitrogenase subunits, and harbored active nitrogenase activity as assessed by acetylene reduction and 15N2incorporation assays. Field experiments in Sierra Mixe using 15N natural abundance or 15N-enrichment assessments over 5 years indicated that atmospheric nitrogen fixation contributed 29%–82% of the nitrogen nutrition of Sierra Mixe maize.

Original languageEnglish (US)
Article numbere2006352
JournalPLoS Biology
Volume16
Issue number8
DOIs
StatePublished - Aug 7 2018

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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    Van Deynze, A., Zamora, P., Delaux, P. M., Heitmann, C., Jayaraman, D., Rajasekar, S., Graham, D., Maeda, J., Gibson, D., Schwartz, K. D., Berry, A. M., Bhatnagar, S., Jospin, G., Darling, A., Jeannotte, R., Lopez, J., Weimer, B. C., Eisen, J. A., Shapiro, H. Y., ... Bennett, A. B. (2018). Nitrogen fixation in a landrace of maize is supported by a mucilage-associated diazotrophic microbiota. PLoS Biology, 16(8), [e2006352]. https://doi.org/10.1371/journal.pbio.2006352