Nitric oxide reaction with lipid peroxyl radicals spares α-tocopherol during lipid peroxidation. Greater oxidant protection from the pair nitric oxide/α-tocopherol than α-tocopherol/ascorbate

Homero Rubbo, Rafael Radi, Daniel Anselmi, Marion Kirk, Stephen Barnes, John Butler, Jason P. Eiserich, Bruce A. Freeman

Research output: Contribution to journalArticle

153 Scopus citations

Abstract

The reactions of nitric oxide (·NO) and α-tocopherol (α-TH) during membrane lipid oxidation were examined and compared with the pair α- TH/ascorbate. Nitric oxide serves as a more potent inhibitor of lipid peroxidation propagation reactions than α-TH and protects α-TH from oxidation. Mass spectrometry, oxygen and ·NO consumption, conjugated diene analyses, and α-TH fluorescence determinations all demonstrated that ·NO preferentially reacts with lipid radical species, with α-TH consumption not occurring until ·NO concentrations fell below a critical level. In addition, α-TH and ·NO cooperatively inhibit lipid peroxidation, exhibiting greater antioxidant capacity than the pair α-TH/ascorbate. Pulse radiolysis analysis showed no direct reaction between ·NO and α-tocopheroxyl radical (α-T·), inferring that peroxyl radical termination reactions are the principal lipid- protective mechanism mediated by ·NO. These observations support the concept that ·NO is a potent chain breaking antioxidant toward peroxidizing lipids, due to facile radical-radical termination reactions with lipid radical species, thus preventing α-TH loss. The reduction of α-T by ascorbate was a comparatively less efficient mechanism for preserving α-TH than ·NO- mediated termination of peroxyl radicals, due to slower reaction kinetics and limited transfer of reducing equivalents from the aqueous phase. Thus, the high lipid/water partition coefficient of ·NO, its capacity to diffuse and concentrate in lipophilic milieu, and a potent reactivity toward lipid radical species reveal how ·NO can play a critical role in regulating membrane and lipoprotein lipid oxidation reactions.

Original languageEnglish (US)
Pages (from-to)10812-10818
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number15
DOIs
StatePublished - Apr 14 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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