Abstract
We investigated the early events involved in loss of mitochondrial membrane potential (ΔΨ mt) leading to apoptosis in cells derived from patients with acute lymphocytic leukemia after exposure to phenolic antioxidants. Using the nitric oxide binding dye diaminofluorescein-FM diacetate, we found that intracellular nitric oxide (NO) levels increased significantly within 4 h after exposure to the antioxidants curcumin, carnosol, and quercetin. Inhibition of nitric oxide synthetase (NOS) activity with mercaptoethylguanidine increased the percentage of leukemia cells with depolarized mitochondria membranes after antioxidant treatment. These data suggest that NO production in the leukemia-derived cells may be a protective response to maintain ΔΨ mt after antioxidant exposure and inhibition of NOS increases the disruption of mitochondrial homeostasis induced by the antioxidants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43-52 |
| Number of pages | 10 |
| Journal | Cancer Letters |
| Volume | 215 |
| Issue number | 1 |
| DOIs | |
| State | Published - Nov 8 2004 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Leukemia
- Mitochondria
- Nitric oxide
- Phenolic antioxidants
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Nitric oxide is synthesized in acute leukemia cells after exposure to phenolic antioxidants and initially protects against mitochondrial membrane depolarization. / Kellner, Christian; Zunino, Susan J.
In: Cancer Letters, Vol. 215, No. 1, 08.11.2004, p. 43-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nitric oxide is synthesized in acute leukemia cells after exposure to phenolic antioxidants and initially protects against mitochondrial membrane depolarization
AU - Kellner, Christian
AU - Zunino, Susan J.
PY - 2004/11/8
Y1 - 2004/11/8
N2 - We investigated the early events involved in loss of mitochondrial membrane potential (ΔΨ mt) leading to apoptosis in cells derived from patients with acute lymphocytic leukemia after exposure to phenolic antioxidants. Using the nitric oxide binding dye diaminofluorescein-FM diacetate, we found that intracellular nitric oxide (NO) levels increased significantly within 4 h after exposure to the antioxidants curcumin, carnosol, and quercetin. Inhibition of nitric oxide synthetase (NOS) activity with mercaptoethylguanidine increased the percentage of leukemia cells with depolarized mitochondria membranes after antioxidant treatment. These data suggest that NO production in the leukemia-derived cells may be a protective response to maintain ΔΨ mt after antioxidant exposure and inhibition of NOS increases the disruption of mitochondrial homeostasis induced by the antioxidants.
AB - We investigated the early events involved in loss of mitochondrial membrane potential (ΔΨ mt) leading to apoptosis in cells derived from patients with acute lymphocytic leukemia after exposure to phenolic antioxidants. Using the nitric oxide binding dye diaminofluorescein-FM diacetate, we found that intracellular nitric oxide (NO) levels increased significantly within 4 h after exposure to the antioxidants curcumin, carnosol, and quercetin. Inhibition of nitric oxide synthetase (NOS) activity with mercaptoethylguanidine increased the percentage of leukemia cells with depolarized mitochondria membranes after antioxidant treatment. These data suggest that NO production in the leukemia-derived cells may be a protective response to maintain ΔΨ mt after antioxidant exposure and inhibition of NOS increases the disruption of mitochondrial homeostasis induced by the antioxidants.
KW - Apoptosis
KW - Leukemia
KW - Mitochondria
KW - Nitric oxide
KW - Phenolic antioxidants
UR - http://www.scopus.com/inward/record.url?scp=4544337595&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4544337595&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2004.06.046
DO - 10.1016/j.canlet.2004.06.046
M3 - Article
C2 - 15374631
AN - SCOPUS:4544337595
VL - 215
SP - 43
EP - 52
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -