TY - JOUR
T1 - Nitric oxide differentially modulates ON and OFF responses of retinal ganglion cells
AU - Wang, Guo Yong
AU - Liets, Lauren C.
AU - Chalupa, Leo M.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Several lines of evidence suggest that nitric oxide (NO) can regulate diverse retinal functions, but whether this gas is capable of modulating the visual responses of retinal output neurons has not been established. In the present study the effects of NO on rod-driven responses of retinal ganglion cells were tested by making whole cell patch-clamp recordings from morphologically identified ganglion cells in the isolated ferret retina. Bath application of L-arginine, the substrate of nitric oxide synthase, and S-nitroso-N-acetylpenicillamine, the NO donor, was found to differentially affect ON and OFF discharge patterns. The introduction of these drugs significantly decreased visual responses of retinal ganglion cells, but the effects were more pronounced on OFF than on ON discharges. The peak discharge rates of ON responses were usually reduced by about 40%, but not completely blocked. In contrast, OFF responses were completely blocked in most cells. These differential effects were observed in ON-OFF cells as well as in cells that yielded just ON or OFF discharges. The OFF responses that were blocked by NO were also blocked by DL-2-amino-phosphonobutyric acid (APB) and strychnine, suggesting the involvement of the APB-sensitive rod pathway.
AB - Several lines of evidence suggest that nitric oxide (NO) can regulate diverse retinal functions, but whether this gas is capable of modulating the visual responses of retinal output neurons has not been established. In the present study the effects of NO on rod-driven responses of retinal ganglion cells were tested by making whole cell patch-clamp recordings from morphologically identified ganglion cells in the isolated ferret retina. Bath application of L-arginine, the substrate of nitric oxide synthase, and S-nitroso-N-acetylpenicillamine, the NO donor, was found to differentially affect ON and OFF discharge patterns. The introduction of these drugs significantly decreased visual responses of retinal ganglion cells, but the effects were more pronounced on OFF than on ON discharges. The peak discharge rates of ON responses were usually reduced by about 40%, but not completely blocked. In contrast, OFF responses were completely blocked in most cells. These differential effects were observed in ON-OFF cells as well as in cells that yielded just ON or OFF discharges. The OFF responses that were blocked by NO were also blocked by DL-2-amino-phosphonobutyric acid (APB) and strychnine, suggesting the involvement of the APB-sensitive rod pathway.
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U2 - 10.1152/jn.00243.2003
DO - 10.1152/jn.00243.2003
M3 - Article
C2 - 12724368
AN - SCOPUS:0041815961
VL - 90
SP - 1304
EP - 1313
JO - Journal of Neurophysiology
JF - Journal of Neurophysiology
SN - 0022-3077
IS - 2
ER -