Abstract
In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Experimental Medicine and Biology |
| Pages | 323-328 |
| Number of pages | 6 |
| Volume | 662 |
| DOIs | |
| State | Published - 2010 |
| Externally published | Yes |
Publication series
| Name | Advances in Experimental Medicine and Biology |
|---|---|
| Volume | 662 |
| ISSN (Print) | 00652598 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. / Masuda, Kazumi; Takakura, Hisashi; Furuichi, Yasuro; Iwase, Satoshi; Jue, Thomas.
Advances in Experimental Medicine and Biology. Vol. 662 2010. p. 323-328 (Advances in Experimental Medicine and Biology; Vol. 662).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle
AU - Masuda, Kazumi
AU - Takakura, Hisashi
AU - Furuichi, Yasuro
AU - Iwase, Satoshi
AU - Jue, Thomas
PY - 2010
Y1 - 2010
N2 - In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.
AB - In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.
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U2 - 10.1007/978-1-4419-1241-1_46
DO - 10.1007/978-1-4419-1241-1_46
M3 - Conference contribution
SN - 9781441912398
VL - 662
T3 - Advances in Experimental Medicine and Biology
SP - 323
EP - 328
BT - Advances in Experimental Medicine and Biology
ER -