NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle

Kazumi Masuda, Hisashi Takakura, Yasuro Furuichi, Satoshi Iwase, Thomas Jue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

15 Scopus citations

Abstract

In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages323-328
Number of pages6
Volume662
DOIs
StatePublished - 2010
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume662
ISSN (Print)00652598

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Masuda, K., Takakura, H., Furuichi, Y., Iwase, S., & Jue, T. (2010). NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. In Advances in Experimental Medicine and Biology (Vol. 662, pp. 323-328). (Advances in Experimental Medicine and Biology; Vol. 662). https://doi.org/10.1007/978-1-4419-1241-1_46