TY - GEN
T1 - NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle
AU - Masuda, Kazumi
AU - Takakura, Hisashi
AU - Furuichi, Yasuro
AU - Iwase, Satoshi
AU - Jue, Thomas
PY - 2010
Y1 - 2010
N2 - In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.
AB - In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.
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U2 - 10.1007/978-1-4419-1241-1_46
DO - 10.1007/978-1-4419-1241-1_46
M3 - Conference contribution
C2 - 20204810
AN - SCOPUS:77949882443
SN - 9781441912398
VL - 662
T3 - Advances in Experimental Medicine and Biology
SP - 323
EP - 328
BT - Advances in Experimental Medicine and Biology
ER -