NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle

Kazumi Masuda, Hisashi Takakura, Yasuro Furuichi, Satoshi Iwase, Thomas Jue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Citations (Scopus)

Abstract

In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (D[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of D[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the D[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages323-328
Number of pages6
Volume662
DOIs
StatePublished - 2010
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume662
ISSN (Print)00652598

Fingerprint

Hindlimb
Muscle
Buffers
Blood
Muscles
Perfusion
Bicarbonates
Muscle Contraction
Workload
Rats
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Masuda, K., Takakura, H., Furuichi, Y., Iwase, S., & Jue, T. (2010). NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. In Advances in Experimental Medicine and Biology (Vol. 662, pp. 323-328). (Advances in Experimental Medicine and Biology; Vol. 662). https://doi.org/10.1007/978-1-4419-1241-1_46

NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. / Masuda, Kazumi; Takakura, Hisashi; Furuichi, Yasuro; Iwase, Satoshi; Jue, Thomas.

Advances in Experimental Medicine and Biology. Vol. 662 2010. p. 323-328 (Advances in Experimental Medicine and Biology; Vol. 662).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Masuda, K, Takakura, H, Furuichi, Y, Iwase, S & Jue, T 2010, NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. in Advances in Experimental Medicine and Biology. vol. 662, Advances in Experimental Medicine and Biology, vol. 662, pp. 323-328. https://doi.org/10.1007/978-1-4419-1241-1_46
Masuda K, Takakura H, Furuichi Y, Iwase S, Jue T. NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. In Advances in Experimental Medicine and Biology. Vol. 662. 2010. p. 323-328. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4419-1241-1_46
Masuda, Kazumi ; Takakura, Hisashi ; Furuichi, Yasuro ; Iwase, Satoshi ; Jue, Thomas. / NIRS measurement of O2 dynamics in contracting blood and buffer perfused hindlimb muscle. Advances in Experimental Medicine and Biology. Vol. 662 2010. pp. 323-328 (Advances in Experimental Medicine and Biology).
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