Niacin attenuates bleomycin-induced lung fibrosis in the hamster.

Q. J. Wang, S. N. Giri, D. M. Hyde, J. M. Nakashima, I. Javadi

Research output: Contribution to journalArticle

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Abstract

Bleomycin (BLM)-induced lung fibrosis has been shown to be accompanied by the activation of poly(ADP-ribose) polymerase and depletion of nicotinamide adenine dinucleotide (NAD) in the lung. Niacin, a precursor of NAD, was used in the present study to investigate its possible ameliorating effect on BLM-induced pulmonary fibrosis in hamsters. Niacin (500 mg/kg IP) or saline (IP) was injected daily for 16 or 23 days. On day 3, hamsters were treated with BLM (7.5 U/5 mL/kg) or an equivalent volume of saline intratracheally. BLM alone significantly increased lung hydroxyproline levels, bronchoalveolar lavage fluid protein concentration, and various inflammatory cell counts in the lavage in both experiments. In addition, BLM alone elevated prolyl hydroxylase and poly(adenosine-5'-diphosphate [ADP]-ribose) polymerase activities in the 3-week study. Niacin treatment significantly decreased BLM-elevated lung hydroxyproline, prolyl hydroxylase, and poly(ADP-ribose) polymerase activities. Histopathology revealed that niacin treatment attenuated BLM-induced thickened alveolar septa, foci of fibrotic consolidation, and accumulations of inflammatory cells in the parenchyma and air spaces. The ability of niacin to attenuate BLM-induced lung fibrosis in hamsters suggests that it may have potential as an antifibrotic agent in humans.

Original languageEnglish (US)
Pages (from-to)13-22
Number of pages10
JournalJournal of Biochemical Toxicology
Volume5
Issue number1
StatePublished - Mar 1990

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Niacin
Bleomycin
Cricetinae
Fibrosis
Lung
Poly Adenosine Diphosphate Ribose
Ribose
Diphosphates
Adenosine
Prolyl Hydroxylases
Hydroxyproline
NAD
Pulmonary Fibrosis
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Consolidation
Cell Count
Chemical activation
Air
Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wang, Q. J., Giri, S. N., Hyde, D. M., Nakashima, J. M., & Javadi, I. (1990). Niacin attenuates bleomycin-induced lung fibrosis in the hamster. Journal of Biochemical Toxicology, 5(1), 13-22.

Niacin attenuates bleomycin-induced lung fibrosis in the hamster. / Wang, Q. J.; Giri, S. N.; Hyde, D. M.; Nakashima, J. M.; Javadi, I.

In: Journal of Biochemical Toxicology, Vol. 5, No. 1, 03.1990, p. 13-22.

Research output: Contribution to journalArticle

Wang, QJ, Giri, SN, Hyde, DM, Nakashima, JM & Javadi, I 1990, 'Niacin attenuates bleomycin-induced lung fibrosis in the hamster.', Journal of Biochemical Toxicology, vol. 5, no. 1, pp. 13-22.
Wang QJ, Giri SN, Hyde DM, Nakashima JM, Javadi I. Niacin attenuates bleomycin-induced lung fibrosis in the hamster. Journal of Biochemical Toxicology. 1990 Mar;5(1):13-22.
Wang, Q. J. ; Giri, S. N. ; Hyde, D. M. ; Nakashima, J. M. ; Javadi, I. / Niacin attenuates bleomycin-induced lung fibrosis in the hamster. In: Journal of Biochemical Toxicology. 1990 ; Vol. 5, No. 1. pp. 13-22.
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