Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

Alex Pauvolid-Corrêa, Owen Solberg, Dinair Couto-Lima, Joan Kenney, Nicolau Serra-Freire, Aaron Brault, Rita Nogueira, Stanley Langevin, Nicholas Komar

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21 Scopus citations

Abstract

We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalArchives of Virology
Volume160
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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    Pauvolid-Corrêa, A., Solberg, O., Couto-Lima, D., Kenney, J., Serra-Freire, N., Brault, A., Nogueira, R., Langevin, S., & Komar, N. (2015). Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil. Archives of Virology, 160(1), 21-27. https://doi.org/10.1007/s00705-014-2219-8