TY - JOUR
T1 - Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil
AU - Pauvolid-Corrêa, Alex
AU - Solberg, Owen
AU - Couto-Lima, Dinair
AU - Kenney, Joan
AU - Serra-Freire, Nicolau
AU - Brault, Aaron
AU - Nogueira, Rita
AU - Langevin, Stanley
AU - Komar, Nicholas
PY - 2015/1/1
Y1 - 2015/1/1
N2 - We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
AB - We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.
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U2 - 10.1007/s00705-014-2219-8
DO - 10.1007/s00705-014-2219-8
M3 - Article
C2 - 25252815
AN - SCOPUS:84925354039
VL - 160
SP - 21
EP - 27
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 1
ER -