Next-generation sequencing technologies

W. Richard McCombie, John D. McPherson, Elaine R. Mardis

Research output: Contribution to journalArticle

Abstract

Although DNA and RNA sequencing has a history spanning five decades, large-scale massively parallel sequencing, or next-generation sequencing (NGS), has only been commercially available for about 10 years. Nonetheless, the meteoric increase in sequencing throughput with NGS has dramatically changed our understanding of our genome and ourselves. Sequencing the first human genome as a haploid reference took nearly 10 years but nowa full diploid human genome sequence can be accomplished in just a fewdays. NGS has also reduced the cost of generating sequence data and a plethora of sequence-based methods for probing a genome have emerged usingNGSas the readout and have been applied to many species. NGS methods have also entered the medical realm and will see an increasing use in diagnosis and treatment. NGS has largely been driven by short-read generation (150 bp) but new platforms have emerged and are now capable of generating long multikilobase reads. These latter platforms enable reference-independent genome assemblies and longrange haplotype generation. Rapid DNA and RNA sequencing is now mainstream and will continue to have an increasing impact on biology and medicine.

Original languageEnglish (US)
Article numbera036798
JournalCold Spring Harbor Perspectives in Medicine
Volume9
Issue number11
DOIs
StatePublished - Nov 2019

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RNA Sequence Analysis
Genes
Genome
Human Genome
Technology
DNA Sequence Analysis
High-Throughput Nucleotide Sequencing
Haploidy
Diploidy
Haplotypes
RNA
History
Medicine
DNA
Costs and Cost Analysis
Throughput
Costs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Next-generation sequencing technologies. / McCombie, W. Richard; McPherson, John D.; Mardis, Elaine R.

In: Cold Spring Harbor Perspectives in Medicine, Vol. 9, No. 11, a036798, 11.2019.

Research output: Contribution to journalArticle

McCombie, W. Richard ; McPherson, John D. ; Mardis, Elaine R. / Next-generation sequencing technologies. In: Cold Spring Harbor Perspectives in Medicine. 2019 ; Vol. 9, No. 11.
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