New Tetrahymena basal body protein components identify basal body domain structure

Chandra L. Kilburn, Chad G. Pearson, Edwin P. Romijn, Janet B. Meehl, Thomas H. Giddings, Brady P. Culver, John R. Yates, Mark Winey

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Basal bodies organize the nine doublet microtubules found in cilia. Cilia are required for a variety of cellular functions, including motility and sensing stimuli. Understanding this biochemically complex organelle requires an inventory of the molecular components and the contribution each makes to the overall structure. We define a basal body proteome and determine the specific localization of basal body components in the ciliated protozoan Tetrahymena thermophila. Using a biochemical, bioinformatic, and genetic approach, we identify 97 known and candidate basal body proteins. 24 novel T. thermophila basal body proteins were identified, 19 of which were localized to the ultrastructural level, as seen by immunoelectron microscopy. Importantly, we find proteins from several structural domains within the basal body, allowing us to reveal how each component contributes to the overall organization. Thus, we present a high resolution localization map of basal body structure highlighting important new components for future functional studies.

Original languageEnglish (US)
Pages (from-to)905-912
Number of pages8
JournalJournal of Cell Biology
Volume178
Issue number6
DOIs
StatePublished - Sep 10 2007
Externally publishedYes

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Basal Bodies
Tetrahymena
Tetrahymena thermophila
Proteins
Cilia
Immunoelectron Microscopy
Proteome
Computational Biology
Microtubules
Organelles
Molecular Biology
Equipment and Supplies

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kilburn, C. L., Pearson, C. G., Romijn, E. P., Meehl, J. B., Giddings, T. H., Culver, B. P., ... Winey, M. (2007). New Tetrahymena basal body protein components identify basal body domain structure. Journal of Cell Biology, 178(6), 905-912. https://doi.org/10.1083/jcb.200703109

New Tetrahymena basal body protein components identify basal body domain structure. / Kilburn, Chandra L.; Pearson, Chad G.; Romijn, Edwin P.; Meehl, Janet B.; Giddings, Thomas H.; Culver, Brady P.; Yates, John R.; Winey, Mark.

In: Journal of Cell Biology, Vol. 178, No. 6, 10.09.2007, p. 905-912.

Research output: Contribution to journalArticle

Kilburn, CL, Pearson, CG, Romijn, EP, Meehl, JB, Giddings, TH, Culver, BP, Yates, JR & Winey, M 2007, 'New Tetrahymena basal body protein components identify basal body domain structure', Journal of Cell Biology, vol. 178, no. 6, pp. 905-912. https://doi.org/10.1083/jcb.200703109
Kilburn CL, Pearson CG, Romijn EP, Meehl JB, Giddings TH, Culver BP et al. New Tetrahymena basal body protein components identify basal body domain structure. Journal of Cell Biology. 2007 Sep 10;178(6):905-912. https://doi.org/10.1083/jcb.200703109
Kilburn, Chandra L. ; Pearson, Chad G. ; Romijn, Edwin P. ; Meehl, Janet B. ; Giddings, Thomas H. ; Culver, Brady P. ; Yates, John R. ; Winey, Mark. / New Tetrahymena basal body protein components identify basal body domain structure. In: Journal of Cell Biology. 2007 ; Vol. 178, No. 6. pp. 905-912.
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