New methods for time-resolved fluorescence spectroscopy data analysis based on the laguerre expansion technique: Applications in tissue diagnosis

Javier A. Jo, Laura Marcu, Q. Fang, T. Papaioannou, J. H. Qiao, M. C. Fishbein, B. Beseth, A. H. Dorafshar, T. Reil, D. Baker, J. Freischlag

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objectives: A new deconvolution method for the analysis of time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) data is introduced and applied for tissue diagnosis. Method: The intrinsic TR-LIFS decays are expanded on a Laguerre basis, and the computed Laguerre expansion coefficients (LEC) are used to characterize the sample fluorescence emission. The method was applied for the diagnosis of atherosclerotic vulnerable plaques. Results: At a first stage, using a rabbit atherosclerotic model, 73 TR-LIFS in-vivo measurements from the normal and atherosclerotic aorta segments of eight rabbits were taken. The Laguerre deconvolution technique was able to accurately deconvolve the TR-LIFS measurements. More interesting, the LEC reflected the changes in the arterial biochemical composition and provided discrimination of lesions rich in macrophages/foam-cells with high sensitivity (> 85%) and specificity (> 95%). At a second stage, 348 TR-LIFS measure-ments were obtained from the explanted carotid arteries of 30 patients. Lesions with significant inflammatory cells (macrophages/foam-cells and lymphocytes) were detected with high sensitivity (> 80%) and specificity (> 90%), using LEC-based classifiers. Conclusion: This study has demonstrated the potential of using TR-LIFS information by means of LEC for in-vivo tissue diagnosis, and specifically for detecting inflammation in atherosclerotic lesions, a key marker of plaque vulnerability.

Original languageEnglish (US)
Pages (from-to)206-211
Number of pages6
JournalMethods of Information in Medicine
Volume46
Issue number2
StatePublished - 2007

Keywords

  • Atherosclerosis
  • Laguerre deconvolution
  • Time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Health Informatics
  • Health Information Management
  • Nursing(all)

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    Jo, J. A., Marcu, L., Fang, Q., Papaioannou, T., Qiao, J. H., Fishbein, M. C., Beseth, B., Dorafshar, A. H., Reil, T., Baker, D., & Freischlag, J. (2007). New methods for time-resolved fluorescence spectroscopy data analysis based on the laguerre expansion technique: Applications in tissue diagnosis. Methods of Information in Medicine, 46(2), 206-211.