New insights into the mechanisms of fertilization: Comparison of the fertilization steps, composition, and structure of the zona pellucida between horses and pigs

Sylvie Mugnier, Maria Elena Dell'Aquila, Jesus Pelaez, Cécile Douet, Barbara Ambruosi, Teresa De Santis, Giovanni Michele Lacalandra, Claude Lebos, Pierre Yves Sizaret, Bernadette Delaleu, Philippe Monget, Pascal Mermillod, Michèle Magistrini, Stuart A Meyers, Ghylène Goudet

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The mechanism of fertilization remains largely enigmatic in mammals. Most studies exploring the molecular mechanism underlying fertilization have been restricted to a single species, generally the mouse, without a comparative approach. However, the identification of divergences between species could allow us to highlight key components in the mechanism of fertilization. In the pig, in vitro fertilization (IVF) and polyspermy rates are high, and spermatozoa penetrate easily through the zona pellucida (ZP). In contrast, IVF rates are low in the horse, and polyspermy is scarce. Our objective was to develop a comparative strategy between these two divergent models. First, we compared the role of equine and porcine gametes in the following five functions using intraspecific and interspecific IVF: ZP binding, acrosome reaction, penetration through the ZP, gamete fusion, and pronucleus formation. Under in vitro conditions, we showed that the ZP is a determining element in sperm-ZP attachment and penetration, whereas the capacity of the spermatozoa is of less importance. In contrast, the capacity of the spermatozoa is a key component of the acrosome reaction step. Second, we compared the composition and structure of the equine and porcine ZP. We observed differences in the number and localization of the ZP glycoproteins and in the mesh-like structure of the ZP between equine and porcine species. These differences might correlate with the differences in spermatozoal attachment and penetration rates. In conclusion, our comparative approach allows us to identify determining elements in the mechanism of fertilization.

Original languageEnglish (US)
Pages (from-to)856-870
Number of pages15
JournalBiology of Reproduction
Volume81
Issue number5
DOIs
StatePublished - 2009

Keywords

  • Acrosome reaction
  • Confocal microscopy
  • Fertilization
  • Gamete biology
  • Gamete interaction
  • ICSI
  • In vitro fertilization
  • Oocyte
  • SEM
  • Spermatozoa
  • TEM
  • Zona pellucida

ASJC Scopus subject areas

  • Cell Biology

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    Mugnier, S., Dell'Aquila, M. E., Pelaez, J., Douet, C., Ambruosi, B., De Santis, T., Lacalandra, G. M., Lebos, C., Sizaret, P. Y., Delaleu, B., Monget, P., Mermillod, P., Magistrini, M., Meyers, S. A., & Goudet, G. (2009). New insights into the mechanisms of fertilization: Comparison of the fertilization steps, composition, and structure of the zona pellucida between horses and pigs. Biology of Reproduction, 81(5), 856-870. https://doi.org/10.1095/biolreprod.109.077651