New disease records for hatchery-reared sturgeon, I, Expansion of frog virus 3 host range into Scaphirhynchus albus

Thomas B. Waltzek, Debra L. Miller, Matthew J. Gray, Bruce Drecktrah, Jeffrey T. Briggler, Beth Macconnell, Crystal Hudson, Lacey Hopper, John Friary, Susan C. Yun, Kirsten V. Malm, E. Scott Weber, Ronald Hedrick

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In 2009, juvenile pallid sturgeon Scaphirhynchus albus, reared at the Blind Pony State Fish Hatchery (Missouri, USA) to replenish dwindling wild stocks, experienced mass mortality. Histological examination revealed extensive necrosis of the haematopoietic tissues, and a virus was isolated from affected organs in cell culture and then observed by electron microscopy. Experimental infection studies revealed that the virus is highly pathogenic to juvenile pallid sturgeon, one of several species of sturgeon currently listed as Endangered. The DNA sequence of the full length major capsid protein gene of the virus was identical to that of the species Frog virus 3 (FV3), the type species for the genus Ranavirus, originally isolated from northern leopard frog Lithobates pipiens. Although FV3 infections and epizootics in amphibians and reptiles are well documented, there is only 1 prior report of a natural infection of FV3 in fish. Our results illustrate the broad potential host range for FV3, with the known potential to cause significant mortality in poikilothermic vertebrates across 3 taxonomic classes including bony fishes, anuran and caudate amphibians, and squamate and testudine reptiles.

Original languageEnglish (US)
Pages (from-to)219-227
Number of pages9
JournalDiseases of Aquatic Organisms
Volume111
Issue number3
DOIs
StatePublished - Jan 1 2014

Fingerprint

Frog virus 3
host range
sturgeon
hatchery
frog
hatcheries
virus
viruses
reptiles
amphibians
Ranavirus
Lithobates
infection
reptile
Squamata
amphibian
coat proteins
fish
frogs
electron microscopy

Keywords

  • Frog virus 3
  • Iridovirus
  • Pallid sturgeon
  • Phylogeny
  • Ranavirus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

New disease records for hatchery-reared sturgeon, I, Expansion of frog virus 3 host range into Scaphirhynchus albus. / Waltzek, Thomas B.; Miller, Debra L.; Gray, Matthew J.; Drecktrah, Bruce; Briggler, Jeffrey T.; Macconnell, Beth; Hudson, Crystal; Hopper, Lacey; Friary, John; Yun, Susan C.; Malm, Kirsten V.; Scott Weber, E.; Hedrick, Ronald.

In: Diseases of Aquatic Organisms, Vol. 111, No. 3, 01.01.2014, p. 219-227.

Research output: Contribution to journalArticle

Waltzek, TB, Miller, DL, Gray, MJ, Drecktrah, B, Briggler, JT, Macconnell, B, Hudson, C, Hopper, L, Friary, J, Yun, SC, Malm, KV, Scott Weber, E & Hedrick, R 2014, 'New disease records for hatchery-reared sturgeon, I, Expansion of frog virus 3 host range into Scaphirhynchus albus', Diseases of Aquatic Organisms, vol. 111, no. 3, pp. 219-227. https://doi.org/10.3354/dao02761
Waltzek, Thomas B. ; Miller, Debra L. ; Gray, Matthew J. ; Drecktrah, Bruce ; Briggler, Jeffrey T. ; Macconnell, Beth ; Hudson, Crystal ; Hopper, Lacey ; Friary, John ; Yun, Susan C. ; Malm, Kirsten V. ; Scott Weber, E. ; Hedrick, Ronald. / New disease records for hatchery-reared sturgeon, I, Expansion of frog virus 3 host range into Scaphirhynchus albus. In: Diseases of Aquatic Organisms. 2014 ; Vol. 111, No. 3. pp. 219-227.
@article{e50b26460b8a4f7fb6e49182ca37d587,
title = "New disease records for hatchery-reared sturgeon, I, Expansion of frog virus 3 host range into Scaphirhynchus albus",
abstract = "In 2009, juvenile pallid sturgeon Scaphirhynchus albus, reared at the Blind Pony State Fish Hatchery (Missouri, USA) to replenish dwindling wild stocks, experienced mass mortality. Histological examination revealed extensive necrosis of the haematopoietic tissues, and a virus was isolated from affected organs in cell culture and then observed by electron microscopy. Experimental infection studies revealed that the virus is highly pathogenic to juvenile pallid sturgeon, one of several species of sturgeon currently listed as Endangered. The DNA sequence of the full length major capsid protein gene of the virus was identical to that of the species Frog virus 3 (FV3), the type species for the genus Ranavirus, originally isolated from northern leopard frog Lithobates pipiens. Although FV3 infections and epizootics in amphibians and reptiles are well documented, there is only 1 prior report of a natural infection of FV3 in fish. Our results illustrate the broad potential host range for FV3, with the known potential to cause significant mortality in poikilothermic vertebrates across 3 taxonomic classes including bony fishes, anuran and caudate amphibians, and squamate and testudine reptiles.",
keywords = "Frog virus 3, Iridovirus, Pallid sturgeon, Phylogeny, Ranavirus",
author = "Waltzek, {Thomas B.} and Miller, {Debra L.} and Gray, {Matthew J.} and Bruce Drecktrah and Briggler, {Jeffrey T.} and Beth Macconnell and Crystal Hudson and Lacey Hopper and John Friary and Yun, {Susan C.} and Malm, {Kirsten V.} and {Scott Weber}, E. and Ronald Hedrick",
year = "2014",
month = "1",
day = "1",
doi = "10.3354/dao02761",
language = "English (US)",
volume = "111",
pages = "219--227",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
number = "3",

}

TY - JOUR

T1 - New disease records for hatchery-reared sturgeon, I, Expansion of frog virus 3 host range into Scaphirhynchus albus

AU - Waltzek, Thomas B.

AU - Miller, Debra L.

AU - Gray, Matthew J.

AU - Drecktrah, Bruce

AU - Briggler, Jeffrey T.

AU - Macconnell, Beth

AU - Hudson, Crystal

AU - Hopper, Lacey

AU - Friary, John

AU - Yun, Susan C.

AU - Malm, Kirsten V.

AU - Scott Weber, E.

AU - Hedrick, Ronald

PY - 2014/1/1

Y1 - 2014/1/1

N2 - In 2009, juvenile pallid sturgeon Scaphirhynchus albus, reared at the Blind Pony State Fish Hatchery (Missouri, USA) to replenish dwindling wild stocks, experienced mass mortality. Histological examination revealed extensive necrosis of the haematopoietic tissues, and a virus was isolated from affected organs in cell culture and then observed by electron microscopy. Experimental infection studies revealed that the virus is highly pathogenic to juvenile pallid sturgeon, one of several species of sturgeon currently listed as Endangered. The DNA sequence of the full length major capsid protein gene of the virus was identical to that of the species Frog virus 3 (FV3), the type species for the genus Ranavirus, originally isolated from northern leopard frog Lithobates pipiens. Although FV3 infections and epizootics in amphibians and reptiles are well documented, there is only 1 prior report of a natural infection of FV3 in fish. Our results illustrate the broad potential host range for FV3, with the known potential to cause significant mortality in poikilothermic vertebrates across 3 taxonomic classes including bony fishes, anuran and caudate amphibians, and squamate and testudine reptiles.

AB - In 2009, juvenile pallid sturgeon Scaphirhynchus albus, reared at the Blind Pony State Fish Hatchery (Missouri, USA) to replenish dwindling wild stocks, experienced mass mortality. Histological examination revealed extensive necrosis of the haematopoietic tissues, and a virus was isolated from affected organs in cell culture and then observed by electron microscopy. Experimental infection studies revealed that the virus is highly pathogenic to juvenile pallid sturgeon, one of several species of sturgeon currently listed as Endangered. The DNA sequence of the full length major capsid protein gene of the virus was identical to that of the species Frog virus 3 (FV3), the type species for the genus Ranavirus, originally isolated from northern leopard frog Lithobates pipiens. Although FV3 infections and epizootics in amphibians and reptiles are well documented, there is only 1 prior report of a natural infection of FV3 in fish. Our results illustrate the broad potential host range for FV3, with the known potential to cause significant mortality in poikilothermic vertebrates across 3 taxonomic classes including bony fishes, anuran and caudate amphibians, and squamate and testudine reptiles.

KW - Frog virus 3

KW - Iridovirus

KW - Pallid sturgeon

KW - Phylogeny

KW - Ranavirus

UR - http://www.scopus.com/inward/record.url?scp=84908048629&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908048629&partnerID=8YFLogxK

U2 - 10.3354/dao02761

DO - 10.3354/dao02761

M3 - Article

C2 - 25320034

AN - SCOPUS:84908048629

VL - 111

SP - 219

EP - 227

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

SN - 0177-5103

IS - 3

ER -