New developments in murine imaging for assessing photoreceptor degeneration in vivo

Marie E Burns, Emily S. Levine, Eric B. Miller, Azhar Zam, Pengfei Zhang, Robert Zawadzki, Edward N Pugh Jr

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Optical Coherence Tomography (OCT) is a powerful clinical tool that measures near infrared light backscattered from the eye and other tissues. OCT is used for assessing changes in retinal structure, including layer thicknesses, detachments and the presence of drusen in patient populations. Our custom-built OCT system for the mouse eye quantitatively images all layers of the neural retinal, the RPE, Bruchs’ membrane and the choroid. Longitudinal assessment of the same retinal region reveals that the relative intensities of retinal layers are highly stable in healthy tissue, but show progressive increases in intensity in a model of retinal degeneration. The observed changes in OCT signal have been correlated with ultrastructural disruptions that were most dramatic in the inner segments and nuclei of the rods. These early changes in photoreceptor structure coincided with activation of retinal microglia, which migrated vertically from the inner to the outer retina to phagocytose photoreceptor cell bodies (Levine et al., Vis Res 102:71–79, 2014). We conclude that quantitative analysis of OCT light scattering signals may be a useful tool for early detection and subcellular localization of cell stress prior to cell death, and for assessing the progression of degenerative disease over time. Future efforts to develop sensitive approaches for monitoring microglial dynamics in vivo may likewise elucidate earlier signs of cellular stress during retinal degeneration.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages269-275
Number of pages7
Volume854
DOIs
StatePublished - Oct 1 2016

Publication series

NameAdvances in Experimental Medicine and Biology
Volume854
ISSN (Print)00652598
ISSN (Electronic)22148019

Keywords

  • Arrestin-1
  • Imaging
  • Microglia
  • Mouse
  • Optical coherence tomography (OCT)
  • Photoreceptor
  • Phototransduction
  • Rod
  • Scanning laser ophthalmoscopy (SLO)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Burns, M. E., Levine, E. S., Miller, E. B., Zam, A., Zhang, P., Zawadzki, R., & Pugh Jr, E. N. (2016). New developments in murine imaging for assessing photoreceptor degeneration in vivo. In Advances in Experimental Medicine and Biology (Vol. 854, pp. 269-275). (Advances in Experimental Medicine and Biology; Vol. 854). Springer New York LLC. https://doi.org/10.1007/978-3-319-17121-0_36