New developments in murine imaging for assessing photoreceptor degeneration in vivo

Marie E Burns, Emily S. Levine, Eric B. Miller, Azhar Zam, Pengfei Zhang, Robert Zawadzki, Edward N Pugh Jr

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Optical Coherence Tomography (OCT) is a powerful clinical tool that measures near infrared light backscattered from the eye and other tissues. OCT is used for assessing changes in retinal structure, including layer thicknesses, detachments and the presence of drusen in patient populations. Our custom-built OCT system for the mouse eye quantitatively images all layers of the neural retinal, the RPE, Bruchs’ membrane and the choroid. Longitudinal assessment of the same retinal region reveals that the relative intensities of retinal layers are highly stable in healthy tissue, but show progressive increases in intensity in a model of retinal degeneration. The observed changes in OCT signal have been correlated with ultrastructural disruptions that were most dramatic in the inner segments and nuclei of the rods. These early changes in photoreceptor structure coincided with activation of retinal microglia, which migrated vertically from the inner to the outer retina to phagocytose photoreceptor cell bodies (Levine et al., Vis Res 102:71–79, 2014). We conclude that quantitative analysis of OCT light scattering signals may be a useful tool for early detection and subcellular localization of cell stress prior to cell death, and for assessing the progression of degenerative disease over time. Future efforts to develop sensitive approaches for monitoring microglial dynamics in vivo may likewise elucidate earlier signs of cellular stress during retinal degeneration.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages269-275
Number of pages7
Volume854
DOIs
StatePublished - Oct 1 2016

Publication series

NameAdvances in Experimental Medicine and Biology
Volume854
ISSN (Print)00652598
ISSN (Electronic)22148019

Fingerprint

Optical tomography
Optical Coherence Tomography
Imaging techniques
Retinal Degeneration
Retinal Photoreceptor Cell Inner Segment
Bruch Membrane
Tissue
Light
Photoreceptor Cells
Choroid
Microglia
Cell death
Phagocytosis
Light scattering
Disease Progression
Retina
Cell Death
Chemical activation
Cells
Infrared radiation

Keywords

  • Arrestin-1
  • Imaging
  • Microglia
  • Mouse
  • Optical coherence tomography (OCT)
  • Photoreceptor
  • Phototransduction
  • Rod
  • Scanning laser ophthalmoscopy (SLO)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Burns, M. E., Levine, E. S., Miller, E. B., Zam, A., Zhang, P., Zawadzki, R., & Pugh Jr, E. N. (2016). New developments in murine imaging for assessing photoreceptor degeneration in vivo. In Advances in Experimental Medicine and Biology (Vol. 854, pp. 269-275). (Advances in Experimental Medicine and Biology; Vol. 854). Springer New York LLC. https://doi.org/10.1007/978-3-319-17121-0_36

New developments in murine imaging for assessing photoreceptor degeneration in vivo. / Burns, Marie E; Levine, Emily S.; Miller, Eric B.; Zam, Azhar; Zhang, Pengfei; Zawadzki, Robert; Pugh Jr, Edward N.

Advances in Experimental Medicine and Biology. Vol. 854 Springer New York LLC, 2016. p. 269-275 (Advances in Experimental Medicine and Biology; Vol. 854).

Research output: Chapter in Book/Report/Conference proceedingChapter

Burns, ME, Levine, ES, Miller, EB, Zam, A, Zhang, P, Zawadzki, R & Pugh Jr, EN 2016, New developments in murine imaging for assessing photoreceptor degeneration in vivo. in Advances in Experimental Medicine and Biology. vol. 854, Advances in Experimental Medicine and Biology, vol. 854, Springer New York LLC, pp. 269-275. https://doi.org/10.1007/978-3-319-17121-0_36
Burns ME, Levine ES, Miller EB, Zam A, Zhang P, Zawadzki R et al. New developments in murine imaging for assessing photoreceptor degeneration in vivo. In Advances in Experimental Medicine and Biology. Vol. 854. Springer New York LLC. 2016. p. 269-275. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-3-319-17121-0_36
Burns, Marie E ; Levine, Emily S. ; Miller, Eric B. ; Zam, Azhar ; Zhang, Pengfei ; Zawadzki, Robert ; Pugh Jr, Edward N. / New developments in murine imaging for assessing photoreceptor degeneration in vivo. Advances in Experimental Medicine and Biology. Vol. 854 Springer New York LLC, 2016. pp. 269-275 (Advances in Experimental Medicine and Biology).
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