New bone formation and bone marrow differentiation induced in rats by extracellular bone matrix implantation: Effect of local preirradiation on the process

I. Bleiberg, M. D. Ricciardone, A Hari Reddi, K. F. McCarthy

Research output: Contribution to journalArticle

10 Scopus citations


Subcutaneous implantation of demineralized diaphyseal rat bone matrix in ACI rats initiates a developmental cascade that results in the formation of new endochondral bone and an associated hematopoietic bone marrow differentiation. Irradiation (1500 rad, 60Co) of the implantation site 24 h prior to implantation suppresses the formation of endochondral bone and bone marrow. All phases of the developmental cascade, including chemotaxis, proliferation, and differentiation, are arrested by the irradiation. Simultaneous implantation with the extracellular matrix of bone marrow, bone, pieces of a four-day-old implant or of thoracic muscle - but not of brain, liver, or spleen tissue - results in the development of endochondral bone and bone marrow at the irradiated site. Concurrent implantation with the extracellular matrix of in vitro growing fibroblasts of marrow or ossicle origin does not restore the developmental cascade.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalExperimental Hematology
Issue number4
StatePublished - 1987
Externally publishedYes


ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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