New alleles of the yeast MPS1 gene reveal multiple requirements in spindle pole body duplication

Amy R. Schutz, Mark Winey

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, the Mps1p protein kinase is critical for both spindle pole body (SPB) duplication and the mitotic spindle assembly checkpoint. The mps1-1 mutation causes failure early in SPB duplication, and because the spindle assembly checkpoint is also compromised, mps1-1 cells proceed with a monopolar mitosis and rapidly lose viability. Here we report the genetic and molecular characterization of mps1-1 and five new temperature-sensitive alleles of MPS1. Each of the six alleles contains a single point mutation in the region of the gene encoding the protein kinase domain. The mutations affect several residues conserved among protein kinases, most notably the invariant glutamate in subdomain III. In vivo and in vitro kinase activity of the six epitope-tagged mutant proteins varies widely. Only two display appreciable in vitro activity, and interestingly, this activity is not thermolabile under the assay conditions used. While five of the six alleles cause SPB duplication to fail early, yielding cells with a single SPB, mps1-737 cells proceed into SPB duplication and assemble a second SPB that is structurally defective. This phenotype, together with the observation of intragenic complementation between this unique allele and two others, suggests that Mps1p is required for multiple events in SPB duplication.

Original languageEnglish (US)
Pages (from-to)759-774
Number of pages16
JournalMolecular Biology of the Cell
Volume9
Issue number4
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Spindle Pole Bodies
Yeasts
Alleles
M Phase Cell Cycle Checkpoints
Genes
Protein Kinases
Saccharomyces cerevisiae Proteins
Mutation
Mutant Proteins
Point Mutation
Mitosis
Epitopes
Molecular Biology
Glutamic Acid
Phosphotransferases
Observation
Phenotype
Temperature

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

New alleles of the yeast MPS1 gene reveal multiple requirements in spindle pole body duplication. / Schutz, Amy R.; Winey, Mark.

In: Molecular Biology of the Cell, Vol. 9, No. 4, 01.01.1998, p. 759-774.

Research output: Contribution to journalArticle

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