Neutrophils contain cholesterol crystals in transfusion-related acute lung injury (TRALI)

Michael Van Ness, Hanne M Jensen, Grete N. Adamson, Patricia E. Kysar, Paul Holland

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: Intracellular components of transfusion-related acute lung injury (TRALI) were investigated by transmission electron microscopy. Methods: The lungs from 2 fatal TRALI cases and 2 controls, previously studied by scanning electron microscopy, were studied by transmission electron microscopy. Morphologic data by light and phase microscopy, along with scanning and transmission electron microscopic observations, were collated. Results: The 2 fatal TRALI cases exhibited dense laminated material within capillaries and postcapillary venules, similar to material identified within their neutrophils when viewed by transmission electron microscopy. This material polarized light and is presumed to be cholesterol crystals. Conclusions: The damage to the pulmonary vascular endothelium in TRALI is related to formation of cholesterol crystals originating within neutrophils.

Original languageEnglish (US)
Pages (from-to)170-176
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume140
Issue number2
DOIs
StatePublished - Aug 2013

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Acute Lung Injury
Neutrophils
Transmission Electron Microscopy
Cholesterol
Light
Lung
Venules
Vascular Endothelium
Electron Scanning Microscopy
Microscopy
Electrons

Keywords

  • Basic science
  • Pulmonary
  • Technique
  • Transfusion medicine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Neutrophils contain cholesterol crystals in transfusion-related acute lung injury (TRALI). / Van Ness, Michael; Jensen, Hanne M; Adamson, Grete N.; Kysar, Patricia E.; Holland, Paul.

In: American Journal of Clinical Pathology, Vol. 140, No. 2, 08.2013, p. 170-176.

Research output: Contribution to journalArticle

Van Ness, Michael ; Jensen, Hanne M ; Adamson, Grete N. ; Kysar, Patricia E. ; Holland, Paul. / Neutrophils contain cholesterol crystals in transfusion-related acute lung injury (TRALI). In: American Journal of Clinical Pathology. 2013 ; Vol. 140, No. 2. pp. 170-176.
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