Neutrophil sequestration and migration in localized pulmonary inflammation: Capillary localization and migration across the interalveolar septum

G. P. Downey, G. S. Worthen, P. M. Henson, D. M. Hyde

Research output: Contribution to journalArticle

149 Scopus citations

Abstract

Recent studies have raised the possibility that biophysical properties of the neutrophil, specifically cell size and deformability, along with the unique hemodynamic properties of the pulmonary microvasculature may be important determinants of neutrophil transit through the lung and lead to significant retention in capillaries. Additionally, accumulating evidence supports the concept that leukocytes may emigrate into inflammatory lesions through the pulmonary capillary wall, quite unlike the situation in the systemic circulation where the predominant site of margination and emigration is the postcapillary venule. To confirm and extend this suggestion, morphometric techniques were employed to determine the location and kinetics of neutrophil movement from the vascular compartment to the alveolar space in response to an inflammatory reaction initiated by local instillation of fragments of the activated form of the fifth component of complement (C5f). We found that the site of neutrophil sequestration and probable migration was almost exclusively the capillary. Neutrophil sequestration appeared first in the capillaries of the interalveolar septae, with negligible accumulation in arterioles and venules (less than that in interalveolar septae by a factor of 104 when corrected for the relevant surface areas). Accumulation in the airspaces lagged behind that in the interalveolar septa by about 60 min, placing an upper time-limit on the emigration process across the epithelial barriers. Interestingly, neutrophils sampled by bronchoalveolar lavage represented only 1 to 2% of those neutrophils shown to be present in the airspaces of the lung by morphometric assessment, suggesting that bronchoalveolar lavage may sample only a subpopulation of the emigrated cells. Additionally, neutrophil accumulation in the terminal airways was also found to occur largely from the adjacent capillaries of the pulmonary circulation. This leukocyte sequestration and probable emigration in capillaries is apparently unique to the lung, and it may reflect the combination of the hydrodynamic and geometric conditions present within the pulmonary capillaries.

Original languageEnglish (US)
Pages (from-to)168-176
Number of pages9
JournalAmerican Review of Respiratory Disease
Volume147
Issue number1
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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