Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque

Pietro Presicce, Paranthaman Senthamaraikannan, Manuel Alvarez, Cesar M. Rueda, Monica Cappelletti, Lisa Miller, Alan H. Jobe, Claire A. Chougnet, Suhas G. Kallapur

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Chorioamnionitis, an infection/inflammation of the fetomaternal membranes, is frequently associated with preterm delivery. The mechanisms of inflammation in chorioamnionitis are poorly understood. We hypothesized that neutrophils recruited to the decidua would be the major producers of proinflammatory cytokines. We injected intra-amniotic (IA) interleukin 1beta (IL-1beta) at ~80% gestation in rhesus macaque monkeys, Macaca mulatta, delivered the fetuses surgically 24 h or 72 h after IA injections, and investigated the role of immune cells in the chorion-amnion decidua. IA IL-1beta induced a robust infiltration of neutrophils and significant increases of proinflammatory cytokines in the chorioamnion decidua at 24 h after exposure, with a subsequent decrease at 72 h. Neutrophils in the decidua were the major source of tumor necrosis factor alpha (TNFalpha) and IL-8. Interestingly, IA IL- 1beta also induced a significant increase in anti-inflammatory indoleamine 2,3-dioxygenase (IDO) expression in the decidua neutrophils. The frequency of regulatory T cells (Tregs) and FOXP3 mRNA expression in the decidua did not change after IA IL-1beta injection. Collectively, our data demonstrate that in this model of sterile chorioamnionitis, the decidua neutrophils cause the inflammation in the gestational tissues but may also act as regulators to dampen the inflammation. These results help to understand the contribution of neutrophils to the pathogenesis of chorioamnionitis-induced preterm labor.

Original languageEnglish (US)
Article number56
JournalBiology of Reproduction
Volume92
Issue number2
DOIs
StatePublished - Feb 1 2015

Keywords

  • Chorioamnionitis
  • Decidua
  • IDO
  • Indoleamine 2,3-dioxygenase
  • Inflammation
  • Neutrophils

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque'. Together they form a unique fingerprint.

  • Cite this

    Presicce, P., Senthamaraikannan, P., Alvarez, M., Rueda, C. M., Cappelletti, M., Miller, L., Jobe, A. H., Chougnet, C. A., & Kallapur, S. G. (2015). Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque. Biology of Reproduction, 92(2), [56]. https://doi.org/10.1095/biolreprod.114.124420